Analyzing the Mechanism and Effect of Acid Protease in Wet blue Bating Process for Leather Production
Analyzing the Mechanism and Effect of Acid Protease in Wet blue Bating Process for Leather Production
复制标题
皮革生产蓝湿皮软化过程中酸性蛋白酶作用机理及效果分析
DOI：
10.34314/jalca.v115i1.1463
复制
发表时间：
2020-01
影响因子：
0.9
通讯作者：
Xiao Jing
中科院分区：
文献类型：
--
作者：
Li Hao;Song Jinzhi;Zhu Deyi;Li Yanchun;Cao Shan;Xiao Jing
近年来，为了减少制革厂和鞣制工段产生的污染，越来越多的制革厂从其他地区的工厂购买蓝湿皮，直接作为成品革生产的原料，从而省去了那些污染严重的前道工序。因此，蓝湿皮软化过程是减少不同地区购买的蓝湿皮差异的必要步骤。本研究分析了不同酸性蛋白酶的特性，以选择适合蓝湿皮软化的蛋白酶。对不同酸性蛋白酶的耐铬性分析表明，曲霉属产生的L1和L4比芽孢杆菌产生的具有更高的耐铬性。 L1和L4对蓝湿皮和胶原蛋白的影响表明，L1具有更优异的性能，其中功能蛋白的分子量为48KD。通过SEM和MCT分析，L1可以成功分散蓝湿皮的胶原纤维。此外，胶原蛋白和弹性蛋白的生物降解率分别为0.006‰和0.5‰。说明在软化过程中酸性蛋白酶主要降解弹性蛋白而不是胶原蛋白，从而保证了生产安全。本研究为酸性蛋白酶在软化过程中的应用提供了重要的参考依据，为酸性蛋白酶在软化过程中的机理研究奠定了基础。
In recent years, in order to reduce the pollution produced in beam-house and tanning sections, more and more tanneries purchase wet blue from other factories in other regions directly used as raw materials for finished leather production thereby those polluted preliminary steps can be eliminated. Therefore, the wet blue bating process is an essential step to minimize the differences of wet blue which are purchased from different regions. In this study, the properties of different acid protease are analyzed for selecting suitable protease used for wet blue bating. The analysis of chromium tolerance of different acid proteases reveals that, L1 and L4 produced from Aspergillus have higher chromium resistance than that of produced from Bacillus. The effect of L1 and L4 on wet blue and collagen shows that the L1 has more excellent performance, in which the molecular weight of functional protein is 48 KD. By SEM and MCT analysis, L1 can successfully disperse the collagen fibers of wet blue. Furthermore, the biodegradation rates of collagen and elastin were 0.006‰ and 0.5‰, respectively. It indicates that the acid protease mainly degraded elastin but not collagen in bating process thereby ensuring production safety. This paper provides the importance references for the application and the basis for the development of mechanism of acid protease in bating process.