Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs).
Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs).
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发表时间：
2003-02
期刊：
影响因子：
3.3
通讯作者：
C. Feschotte;L. Swamy;S. Wessler
中科院分区：
文献类型：
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作者：
C. Feschotte;L. Swamy;S. Wessler
偷渡者是一个微型反向重复序列转座元件超家族，广泛存在于植物基因组中。然而，像其他螨类一样，人们对其来源和放大方式知之甚少。有几条证据表明，像水手一样的元素(MLE)是非自治的Stowaway螨类的自主伙伴。为了更好地理解这种关系，我们利用两个水稻亚种几乎完整的基因组序列，生成了几乎所有MLEs和Stowaway家族共存于单个植物物种的第一份清单。34个不同的MLE被发现归类为三个主要支系和25个家族。超过22,000只偷渡螨类被鉴定出来，并归入36个科。在详细的序列比较的基础上，MLE被确定为斯道威螨类最好的候选自主元件。然而，令人惊讶的是，MLE和Stowaway家族之间的序列相似性仅限于末端反向重复序列(TIR)，在少数情况下，仅限于相邻的亚末端序列。这些数据提出了一种模型，在该模型中，水稻中的大多数偷渡螨类被远缘相关元件编码的MLE转座酶交叉动员。
Stowaway is a superfamily of miniature inverted repeat transposable elements (MITEs) that is widespread and abundant in plant genomes. Like other MITEs, however, its origin and mode of amplification are poorly understood. Several lines of evidence point to plant mariner-like elements (MLEs) as the autonomous partners of the nonautonomous Stowaway MITEs. To better understand this relationship, we have taken advantage of the nearly complete genome sequences of two rice subspecies to generate the first inventory of virtually all MLEs and Stowaway families coexisting in a single plant species. Thirty-four different MLEs were found to group into three major clades and 25 families. More than 22,000 Stowaway MITEs were identified and classified into 36 families. On the basis of detailed sequence comparisons, MLEs were confirmed to be the best candidate autonomous elements for Stowaway MITEs. Surprisingly, however, sequence similarity between MLE and Stowaway families was restricted to the terminal inverted repeats (TIRs) and, in a few cases, to adjacent subterminal sequences. These data suggest a model whereby most of the Stowaway MITEs in rice were cross-mobilized by MLE transposases encoded by distantly related elements.