Specific estrogen receptors in the lactating mammary gland of the rat.
Specific estrogen receptors in the lactating mammary gland of the rat.
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大鼠泌乳乳腺中的特定雌激素受体。
DOI：
10.1021/bi00740a023
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发表时间：
1973
期刊：
影响因子：
2.9
通讯作者：
J. Wittliff
中科院分区：
文献类型：
--
作者：
D. Gardner;J. Wittliff
在Fischer大鼠哺乳期乳腺上清液中检测到17p - estrao1特异性受体，其在蔗糖梯度下的沉降系数为8-9 S。根据竞争研究判断，这些受体对雌激素具有特异性，并且对17p-雌二醇具有异常高的亲和力（K d M）。它们本质上是蛋白质，当在蔗糖S特异性受体17p-estradio1上分离时解离成更小的结合成分，在8-9 S沉积，现在已经在子宫的细胞质部分中被描述（Toft和Gorski, 1966； Jensen等）。垂体前叶（Notides, 1970），以及多种乳腺癌，包括动物（Jungblut et al., 1967; Kyser, 1970; McGuire et al., 1971; Wittliff et al., 1972a）和人类起源（Jensen et al., 1972a）。, 1971;Wittliff /。, 1972 b)。然而，尽管正常乳腺组织对雌激素激素的反应性是众所周知的，但在正常乳腺中有类似的雌激素受体的证明却有些难以捉摸。虽然Puca和Bresciani（1969）表明小鼠的静止乳腺组织能够在体外以特定的方式积累[3H]-17/3-雌二醇，但在分子水平上没有提供有关受体机制特征的信息。最近，该实验室（Wittliff et al., 1972a）证实在大鼠哺乳期乳腺的细胞质部分中存在一种特定的雌激素结合成分。该受体在蔗糖梯度上沉积1 ~ 8 S，对17p-雌二醇具有高特异性和亲和力（K d lop9 M）。本文对乳腺中的雌激素结合蛋白进行了更广泛的表征，并为该受体在体内可能的生理作用提供了证据。来自罗切斯特大学医学和牙科学院生物化学系和肿瘤学系，罗切斯特，纽约14642。1973年3月9日收到。这些研究的初步报告于1972年6月18日至24日在华盛顿特区举行的第四届国际内分泌学大会上发表。部分由美国公共卫生服务拨款CA-11198和CA-12836以及欧文·斯特拉斯堡纪念医疗基金会支持。由美国公共卫生服务一般研究支持基金资助的医学院学生研究员。1 estra -1,3,5(IO)-三烯-3,17@-diol。[3090 . 90]我要把我的头发剪掉，我要把它剪掉。12, n, 0。3塔克，H. A.，拉尔森，B. L.，戈尔斯基，J. (1971), EndoTurkington， R. W., Juergens， W. G., Topper, Y .。J. Webb， J. M.和Levy， H. B. (1955)， J. Bid。Chem. 123, Wilkman， J.和Davis， J. W. (1968), Fed. Proc, Fed. american。犯罪学89,152。（1967），内分泌学80,11 39。
Specific receptors of 17P-estradio1, which exhibited sedimentation coefficients of 8-9 S in sucrose gradients, were detected in the 105,OOOg supernatants of the lactating mammary gland of the Fischer rat. These receptors were specific for estrogens, as judged by competition studies, and demonstrated exceptionally high affinity for 17p-estradiol ( K d M). They were protein in nature and dissociated into smaller binding components when separated on sucrose S pecific receptors for 17p-estradio1, sedimenting at 8-9 S, have now been described in the cytoplasmic fraction from the uterus (Toft and Gorski, 1966; Jensen et a/., 1967a), anterior pituitary (Notides, 1970), and a variety of breast carcinomas, both of animal (Jungblut et al., 1967; Kyser, 1970; McGuire et al., 1971; Wittliff et al., 1972a) and human origin (Jensen et ul., 1971; Wittliff et a/., 1972b). However, demonstration of a comparable estrogen receptor in normal mammary gland has been somewhat more elusive, in spite of the well known responsiveness of this tissue to estrogenic hormones. While Puca and Bresciani (1969) showed that quiescent mammary tissue of the mouse was capable of accumulating [ 3H]-17/3-estradiol in vitro in a specific fashion, information regarding the characteristics of a receptor mechanism at the molecular level was not presented. Recently, this laboratory (Wittliff et al., 1972a) demonstrated the presence of a specific estrogen-binding component in the cytosol fraction of the lactating mammary gland of the rat. This receptor, which sedimented a t -8 S on sucrose gradients, was observed to possess high specificity and affinity ( K d lop9 M) for 17p-estradiol. This paper presents a more extensive characterization of the estrogen-binding protein in the mammary gland and offers evidence for a probable physiological role for the receptor in vivo. t From the Department of Biochemistry and the Division of Oncology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642. Receiced March 9, 1973. A preliminary report of these studies was presented at the IVth International Congress of Endocrinology, Washington, D. C., June 18-24, 1972. Supported i n part by U. S. Public Health Service Grants CA-11198 and CA-12836 and the Irwin Strasburger Memorial Medical Foundation. $ Medical student Research Fellow supported by U. S. Public Health Service General Research Support grant. I Estra-1,3,5( IO)-triene-3,17@-diol. 3090 B I O C H E M I S T R Y , V O L . 1 2 , N O . 1 6 , 1 9 7 3 Tucker, H. A., Larson, B. L., and Gorski, J. (1971), EndoTurkington, R. W., Juergens, W. G., and Topper, Y . J. Webb, J. M., and Levy, H. B. (1955), J. Bid . Chem. 123, Wilkman, J., and Davis, J. W. (1968), Fed. Proc., Fed. Amer. crinology 89, 152. (1 967), Endocrinology 80, 11 39.