Deep RNA Sequencing Reveals Complex Transcriptional Landscape of a Bat Adenovirus
Deep RNA Sequencing Reveals Complex Transcriptional Landscape of a Bat Adenovirus
复制标题
深度 RNA 测序揭示了蝙蝠腺病毒的复杂转录景观
DOI：
10.1128/jvi.02332-12
复制
发表时间：
2013-01-01
影响因子：
5.4
通讯作者：
Shi, Zhengli
中科院分区：
文献类型：
--
作者：
Wu, Lijun;Zhou, Peng;Shi, Zhengli
蝙蝠腺病毒是最近发现的一组腺病毒（AdVs），它们在蝙蝠中高度流行，但与来自其他物种的已知AdVs具有低相似性。在这项研究中，使用深度RNA测序分析了来自一种肌炎蝙蝠的肾细胞系感染蝙蝠AdV后五个时间点的转录组。AdV复制的证据是，在6 h时，病毒rna的比例为0.01%，在18 h时为1.3%。进一步分析病毒时间基因表达，发现三个复制阶段，早期基因主要编码宿主相互作用蛋白，中期基因编码DNA复制和组装蛋白，晚期基因编码大多数结构蛋白。几种蝙蝠AdV基因的表达阶段与先前报道的人类AdV 2型对应基因的表达阶段不同。此外，还完全确定了多个基因的单碱基分辨率剪接位点和所有30个病毒基因的启动子区域。同时，确定了时间细胞基因表达谱。差异表达基因中最具代表性的功能类别与细胞免疫应答、转录、翻译、DNA复制和修复有关。总之，深度RNA测序提供了新的蝙蝠AdV和病毒-宿主相互作用的全局转录谱，这将有助于在未来的研究中理解和研究AdV的复制、发病机制和特定的病毒-蝙蝠相互作用。
ABSTRACT Bat adenoviruses are a group of recently identified adenoviruses (AdVs) which are highly prevalent in bats yet share low similarity to known AdVs from other species. In this study, deep RNA sequencing was used to analyze the transcriptome at five time points following the infection of a bat AdV in a kidney cell line derived from a myotis bat species. Evidence of AdV replication was observed with the proportion of viral RNAs ranging from 0.01% at 6 h to 1.3% at 18 h. Further analysis of viral temporal gene expression revealed three replication stages, the early-stage genes encoding mainly host interaction proteins, the intermediate-stage genes for the DNA replication and assembly proteins, and the late-stage genes for most structural proteins. Several bat AdV genes were expressed at stages that differed from those of their counterpart genes previously reported for human AdV type 2. In addition, single-base resolution splice sites of several genes and promoter regions of all 30 viral genes were fully determined. Simultaneously, the temporal cellular gene expression profiles were identified. The most overrepresented functional categories of the differentially expressed genes were related to cellular immune response, transcription, translation, and DNA replication and repair. Taken together, the deep RNA sequencing provided a global, transcriptional profile of the novel bat AdV and the virus-host interactions which will be useful for the understanding and investigation of AdV replication, pathogenesis, and specific virus-bat interactions in future research.