Regulated endocytic routing modulates wingless signaling in Drosophila embryos
Regulated endocytic routing modulates wingless signaling in Drosophila embryos
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DOI：
10.1016/s0092-8674(01)00375-0
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发表时间：
2001-06-01
期刊：
影响因子：
64.5
通讯作者：
Vincent, JP
中科院分区：
文献类型：
--
作者：
Dubois, L;Lecourtois, M;Vincent, JP
胚胎已经进化出各种策略来限制分泌信号的作用。使用HRP-Wingless融合蛋白来跟踪内吞Wingless的命运，我们表明通过靶向溶酶体降解是这样一种策略。无翅蛋白在无翅转录的每个条带的后部特异性降解，即使在过度表达的条件下。如果溶酶体降解受到遗传或化学影响，多余的Wingless积累和异位信号增强。在野生型中，无翅的退化在前部比在后部慢。这部分是由于表皮生长因子受体对信号传导的节段性激活，这加速了后部的无翼降解，从而导致沿着前后轴的不对称无翼信号传导。
Embryos have evolved various strategies to confine the action of secreted signals. Using an HRP-Wingless fusion protein to track the fate of endocytosed Wingless, we show that degradation by targeting to lysosomes is one such strategy. Wingless protein is specifically degraded at the posterior of each stripe of wingless transcription, even under conditions of over-expression. If lysosomal degradation is compromised genetically or chemically, excess Wingless accumulates and ectopic signaling ensues. In the wild-type, Wingless degradation is slower at the anterior than at the posterior. This follows in part from the segmental activation of signaling by the epidermal growth factor receptor, which accelerates Wingless degradation at the posterior, thus leading to asymmetrical Wingless signaling along the anterior-posterior axis.