Inter-specific relationships and hierarchical spatial genetic structuring in Nervilia nipponica, an endangered orchid in Japan
Inter-specific relationships and hierarchical spatial genetic structuring in Nervilia nipponica, an endangered orchid in Japan
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DOI：
10.1007/s10265-010-0314-9
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
S. Gale;Ayako Maeda;Chen-I Chen-Chen-I-Chen-120897107;T. Yukawa
中科院分区：
文献类型：
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作者：
S. Gale;Ayako Maeda;Chen-I Chen-Chen-I-Chen-120897107;T. Yukawa
在规划保护濒危植物物种的措施时，需要了解生殖战略和种子传播在多大程度上导致空间遗传多样性的结构化。在这项研究中，遗传结构的濒危陆生植物Nervilia nipponica进行了调查，使用扩增片段长度多态性，广泛的采样在整个物种的范围在日本和密集的采样在一个单一的人口。有限的多样性被发现在物种作为一个整体，但显着的结构之间的人口。一个基因型是共同的两个相距甚远的网站，可能表明远距离传播。重要的结构也被检测到在密集采样的网站，由于存在两个不同的推定克隆。这些发现与观察到的该物种自花授粉结籽和无性繁殖的能力相一致。鉴于推断该物种具有强大的殖民能力，在制定保护策略时应重点关注识别和确保有利于种子萌发和幼苗建立的栖息地条件。目前，N。nipponicais包括两个多系分类群，都是日本特有的，都不同于N. taiwaniana，一个物种，有些作者认为同种。
An understanding of the extent to which reproductive strategy and seed dispersal lead to the structuring of genetic diversity in space is required when planning measures towards the conservation of endangered plant species. In this study, genetic structure in the endangered terrestrial orchidNervilia nipponicawas investigated using amplified fragment length polymorphisms following extensive sampling throughout the species’ range in Japan and intensive sampling at a single population. Limited diversity was found within the species as a whole, but significant structuring was detected between populations. One genotype was common to two widely separated sites, possibly indicative of long-range dispersal. Significant structure was also detected at the intensively sampled site, as a result of the presence of two distinct putative clones. These findings are consistent with observations of the species’ ability to set seed autogamously and propagate vegetatively. Given the strong colonising capability inferred for the species, attention should focus on identifying and securing habitat conditions conducive to seed germination and seedling establishment in the development of a conservation strategy. As presently circumscribed,N. nipponicais shown to comprise two polyphyletic taxa, both endemic to Japan, and both distinct fromN. taiwaniana, a species that some authors have considered conspecific.