The Studies on Hemin as Mimetic Peroxidase in Salt Bridge Supported Bilayer Lipid Membrane System
The Studies on Hemin as Mimetic Peroxidase in Salt Bridge Supported Bilayer Lipid Membrane System
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DOI：
10.1002/1521-4109(200111)13:17
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发表时间：
2001-11
期刊：
影响因子：
3
通讯作者：
Yan-Li Zhang;Chun-Xu Zhang;Han-xi Shen
中科院分区：
文献类型：
--
作者：
Yan-Li Zhang;Chun-Xu Zhang;Han-xi Shen
氯化血红素在盐桥支持的双层脂膜（sb-BLM）体系中，在pH 7-8时表现出类似过氧化物酶的活性。在sb-BLM上引入月桂酸阴离子（LA），通过正电荷氯高铁血红素和负电荷LA之间的静电作用，氯高铁血红素也被吸附到sb-BLM上，从而增强了氯高铁血红素与电极表面的电子转移。即使在不存在介体（氢供体）的情况下，也观察到氯化血红素的不可逆循环伏安（CV）行为。构建了一种新型的生物相容性sb-BLM传感器。用该传感器研究了氯化血红素的电催化动力学行为。在过氧化氢存在下，氯化血红素的阴极峰电流（IPc）增加，其强度与过氧化氢的浓度成正比。离子强度和pH值对生物传感器的响应的影响进行了研究，以获得最佳的分析性能。
Hemin exhibits mimetic peroxidase activity at pH 7–8 in a salt bridge supported bilayer lipid membrane (sb-BLM) system. sb-BLM was modified with anionic site-lauric acid anions (LA), then, hemin was also adsorbed and incorporated in sb-BLM by electrostatic force between positively charged hemin and negatively charged LA, which results in the enhancement of the electron transfer between hemin and the electrode surface. An irreversible cyclic voltammetry (CV) behavior of hemin was observed even in the absence of mediators (hydrogen donor). A novel and biological compatible sb-BLM biosensor was constructed. The electrocatalytically kinetic behavior of hemin was investigated with this kind of biosensor. The cathodic peak current (IPc) of hemin was increased in the presence of hydrogen peroxide, the intensity of which is in proportion to the concentration of hydrogen peroxide. The effects of ionic strength and pH on the response of the biosensor were also investigated for optimum analytical performance.