Metabolic control analysis. The effects of high enzyme concentrations.
Metabolic control analysis. The effects of high enzyme concentrations.
复制标题
代谢控制分析。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
H. Sauro
中科院分区：
文献类型：
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作者：
D. Fell;H. Sauro
关于酶在途径中与其底物浓度相当的存在是否会影响控制系数的值和代谢控制分析的定理，文献中给出了不同的观点。在这里，我们支持其中一种观点：除非酶隔离含有保守部分的底物，否则没有效果。在这种特殊情况下，我们推导出估计这种效应是否具有显著程度的一般准则，以及确定通量控制系数变化的方程。用数值模拟说明了这一现象的性质和方程的应用。
Differing views have been given in the literature as to whether the presence in a pathway of an enzyme at a concentration comparable to that of its substrate affects the values of control coefficients and the theorems of metabolic control analysis. Here we argue in favour of one of those views: that there is no effect unless the enzyme sequesters a substrate that contains a conserved moiety. In this particular case, we derive both a general criterion for estimating whether such an effect will be of a significant magnitude, and equations for determining the changes in the flux control coefficients. The nature of the phenomenom and the application of the equations are illustrated with a numerical simulation.