Clearance from the vascular compartment of endogenously labelled plasma fibronectin.
Clearance from the vascular compartment of endogenously labelled plasma fibronectin.
复制标题
内源标记的血浆纤连蛋白从血管室中清除。
DOI：
10.1097/00005373-198402000-00001
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Saba,TM
中科院分区：
文献类型：
--
作者：
Deno,DC;Lewis,EP;Saba,TM
血浆纤维连接蛋白是一种大分子量的糖蛋白，可能具有调理和结构粘附作用。纤连蛋白缺乏症已被记录在患者中，特别是创伤或烧伤后早期以及损伤后脓毒症期间。在这项研究中，纤维连接蛋白从血液中的消失进行了研究，在大鼠利用血浆纤维连接蛋白代谢标记的75硒-硒代蛋氨酸。在注射75 Se-硒代蛋氨酸后，在4小时观察到内源性标记的血浆纤连蛋白的最大比活性。此后，它以非单指数的方式与前体的耗尽相关联地下降。用明胶-琼脂糖亲和层析法从供体大鼠血浆中纯化~（75）Se标记的纤维连接蛋白。它保留了其电泳迁移率，明胶粘附，调理活性类似于未标记的血浆纤连蛋白。静脉注射~（75）Se后，血浆中纤维连接蛋白的消失有两个阶段。第一个是少量纤连蛋白的初始快速消失，反映了血浆和间质室之间的分布。第二个是一个较慢的消失期，半衰期（T1/2）至少为15小时。输注明胶包被的颗粒，这是快速清除的RE细胞在肝脏和脾脏，增强75硒纤维连接蛋白从血浆中的消失。这些数据表明纤连蛋白从血管间隙消失的正常速率是相当快的。利用这种实验方法可能会提供有价值的数据纤维连接蛋白动力学的创伤和烧伤的影响。
Plasma fibronectin is a large molecular weight glycoprotein which may have both opsonic and structural adhesive roles. Fibronectin deficiency has been documented in patients especially early after trauma or burn as well as during sepsis following injury. In this study, the disappearance of fibronectin from the blood was studied in rats utilizing plasma fibronectin metabolically labelled with 75 Se-selenomethionine. After injection of 75 Se-selenomethionine, the maximum specific activity of endogenously labelled plasma fibronectin was observed at 4 hours. Thereafter, it declined in a non-monoexponential fashion in association with depletion of the precursor. Labelled 75 Se fibronectin was purified from donor rat plasma by gelatin-sepharose affinity chromatography. It retained its electrophoretic mobility, gelatin adherence, and opsonic activity similar to that of unlabelled plasma fibronectin. Following intravenous injection of 75 Se plasma fibronectin, its disappearance from plasma manifested two phases. The first was an initial fast disappearance of a small amount of fibronectin, reflecting distribution between plasma and interstitial compartments. The second was a slower disappearance phase with a half-time (T 1/2) of at least 15 hours. Infusion of gelatin-coated particles, which are rapidly cleared by RE cells in the liver and spleen, enhanced the disappearance of 75 Se fibronectin from the plasma. These data suggest that the normal rate of fibronectin disappearance from the vascular space is quite fast. Utilization of this experimental approach may provide valuable data on fibronectin kinetics as influenced by trauma and burn.