CHANGES IN MITOTIC RATE AND CELL-CYCLE FRACTIONS CAUSED BY DELAYED FIXATION
CHANGES IN MITOTIC RATE AND CELL-CYCLE FRACTIONS CAUSED BY DELAYED FIXATION
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DOI：
10.1016/0046-8177(90)90030-9
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发表时间：
1990-07-01
期刊：
影响因子：
3.3
通讯作者：
BUDACH, V
中科院分区：
文献类型：
--
作者：
DONHUIJSEN, K;SCHMIDT, U;BUDACH, V
恶性肿瘤的有丝分裂频率和流式细胞术数据对诊断和预后均有重要意义。目前尚不清楚这些因素在多大程度上受到肿瘤活检固定延迟的影响。因此，我们研究了固定5分钟和活检后3、6、9和12小时的人软组织肉瘤异种移植瘤的有丝分裂活性和DNA含量。平均而言，两个观察者计数的有丝分裂数在3h后分别比初始值低13%和10%，12h后分别减少46%和39%。有丝分裂的减少与单个肿瘤的有丝分裂活动程度有关，在有丝分裂比率最低的肉瘤中最小。这些结果是可重复性的。然而，观察到大量固缩的有丝分裂图形，因此计数的减少很大程度上是由于它们的可识别性降低，仅部分归因于细胞周期的完成。保存完好的有丝分裂图在12小时后可见，似乎表明在未固定的活检中，增殖活性只是逐渐减少。流式细胞术的数据没有实质性的变化；仅观察到G2+M期比例的轻微增加。结果得出的一般结论受到这样一个事实的限制，即所研究的肉瘤比大多数癌症具有更高的有丝分裂活性。然而，为了准确地测量恶性程度的有丝分裂计数，早期固定活检是可取的。
The mitosis frequency and flow cytometric data of malignant neoplasms are important, both for diagnosis and for prognosis. It is unclear to what extent these factors are affected by a delay in the fixation of tumor biopsies. We have thus studied the mitotic activity and DNA content in human soft-tissue sarcoma xenotransplants, fixed for periods of 5 minutes and 3, 6, 9 and 12 hours after biopsy. On average, the mitoses counted by two observers were 13% and 10% below initial values after 3 hours, and decreased by 46% and 39% after 12 hours. The mitosis decrease was related to the degree of mitotic activity of individual tumors, and was minimal in the sarcomas with the lowest mitotic rate. These results were reproducible. However, numerous pyknotic mitotic figures were observed, so the decrease in counts is largely due to their reduced identifiability, and only partly attributable to a completion of the cell cycle. Well-preserved mitotic figures demonstrable after 12 hours appear to indicate that the proliferation activity only gradually decreases in unfixed biopsies. The flow cytometric data did not change substantially; only a slight increase in the G2+ M-phase fraction was observed. General conclusions from the results are limited by the fact that the investigated sarcomas had a higher mitotic activity than most carcinomas. Nevertheless, early fixation of biopsies is desirable to accurately measure mitosis counts for the grading of malignancy.