Supercritical CO2 assisted preparation of chitosan-based nano-in-microparticles with potential for efficient pulmonary drug delivery
Supercritical CO2 assisted preparation of chitosan-based nano-in-microparticles with potential for efficient pulmonary drug delivery
复制标题
超临界二氧化碳辅助制备基于壳聚糖的纳米微粒，具有高效肺部药物输送的潜力
DOI：
10.1016/j.jcou.2021.101486
复制
发表时间：
2021-02-23
影响因子：
7.7
通讯作者：
Yao, Shan-Jing
中科院分区：
文献类型：
--
作者：
Peng, Hu-Hong;Wang, Zi-Dan;Yao, Shan-Jing
用于吸入性肺癌治疗的纳米微粒联合收割机结合了空气动力学直径为1-5 μ m的微粒的优点。纳米颗粒在肺中的停留时间长，粘液穿透和癌细胞吸收效率高。本研究采用改进的超临界CO2辅助雾化技术（SAA-HCM）制备了由壳聚糖纳米粒和甘露醇组成的纳米微粒。考察了CO2/液体质量流量比、总质量浓度、纳米粒子/甘露醇比例等工艺参数对纳米粒子尺寸和形貌的影响。定义明确的球形纳米微粒与理论质量中位数的空气动力学直径为1-2？在优化的条件下得到了m。将约40%的纳米颗粒部分以10：90的纳米颗粒/甘露醇比率再分散在去离子水中。同时，在模拟肺中高湿度的条件下观察到纳米微粒中的纳米微粒的快速崩解。综上所述，根据颗粒形成机理，纳米颗粒中的纳米颗粒的结构决定了纳米颗粒的解离和纳米颗粒的再结晶。本工作将拓展SAA-HCM在纳米混悬液制备纳米微粒中的应用，为设计具有精细结构的微粒提供更多的参考。
Nano-in-microparticles used for inhalation lung cancer therapy combine the advantages of microparticles with aerodynamic diameters of 1-5 ?m for deep lung deposition and nanoparticles for long residence time in lung, high mucus penetrating and cancer cells uptake efficiency. In this work, nano-in-microparticles composed of chitosan nanoparticles and mannitol were prepared using a modified supercritical CO2 assisted atomization (SAA-HCM) technique. Influences of process parameters of CO2/liquid mass flow ratio, total mass concentration and nanoparticles/mannitol ratio on size and morphologies of nano-in-microparticles were investigated in detail. Well-defined spherical nano-in-microparticles with theoretical mass median aerodynamic diameter of 1-2 ?m were obtained under optimized conditions. Fraction of about 40 % of nanoparticles was redispersed in deionized water at nanoparticles/mannitol ratio of 10:90. Meanwhile, quick disintegration of nano-in-microparticles was observed under conditions simulating high humidity in lung. In summary, dissociation of nano-in-microparticles and redispersibility of nanoparticles were determined by the structure of nano-in-microparticles according to particles formation mechanism. This work will expand the applicability of SAA-HCM to the preparation of nanoin-microparticles from nanosuspension and provide more references for designing of particles with elaborate structures.