2D IR spectroscopy using four-wave mixing, pulse shaping, and IR upconversion: a quantitative comparison.
2D IR spectroscopy using four-wave mixing, pulse shaping, and IR upconversion: a quantitative comparison.
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DOI：
10.1021/jp312817t
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发表时间：
2013-07-25
影响因子：
2.9
通讯作者：
Cheatum, Christopher M.
中科院分区：
文献类型：
--
作者：
Rock, William;Li, Yun-Liang;Pagano, Philip;Cheatum, Christopher M.
最近的技术进步已经导致用于收集2D IR光谱的设备发生重大变化。脉冲整形提供了几个优点，包括快速的数据收集，固有的相位稳定性和相位循环能力。通过上转换的可见光阵列检测允许使用比IR检测器更便宜、更快、更灵敏且噪声更小的可见光检测器。然而，尽管有这些优势，许多研究人员不愿意实施这些技术。在这里，我们提出了一个定量研究的S/N的二维红外光谱收集与传统的四波混频（FWM）设备，与脉冲整形设备，并通过上转换可见光检测，以解决问题的弱发色团在低浓度下是否仍然可以访问这样的设备。我们发现，增强的平均能力的脉冲整形装置，使小信号的检测，这将是具有挑战性的，即使与传统的FWM装置测量，我们证明了这种能力的氰化二氢叶酸还原酶（DHFR）的样品。
Recent technological advances have led to major changes in the apparatuses used to collect 2D IR spectra. Pulse shaping offers several advantages including rapid data collection, inherent phase stability, and phase cycling capabilities. Visible array detection via upconversion allows the use of visible detectors that are cheaper, faster, more sensitive, and less noisy than IR detectors. However, despite these advantages, many researchers are reluctant to implement these technologies. Here we present a quantitative study of the S/N of 2D IR spectra collected with a traditional four-wave mixing (FWM) apparatus, with a pulse shaping apparatus, and with visible detection via upconversion to address the question of whether or not weak chromophores at low concentrations are still accessible with such an apparatus. We find that the enhanced averaging capability of the pulse shaping apparatus enables the detection of small signals that would be challenging to measure even with the traditional FWM apparatus, and we demonstrate this ability on a sample of cyanylated dihydrofolate reductase (DHFR).
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影响因子：
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作者：
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通讯作者：
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