Visualizing water molecule distribution by atomic force microscopy
Visualizing water molecule distribution by atomic force microscopy
复制标题
DOI：
10.1063/1.3408289
复制
发表时间：
2010-05-21
影响因子：
4.4
通讯作者：
Yamada, Hirofumi
中科院分区：
文献类型：
--
作者：
Kimura, Kenjiro;Ido, Shinichiro;Yamada, Hirofumi
生物分子表面的水化结构对于理解生物分子的各种生物功能和稳定性的机制是必不可少的。在这里，我们演示了使用配备低噪声偏转传感器的原子力显微镜系统来测量局部水化结构。我们将这种方法应用于白云母/水界面的分析，并成功地可视化了晶体上特定位置的水化结构。此外，在生物分子/缓冲溶液界面，我们发现表面水化层比白云母/水界面更紧密。(C)2010年美国物理研究所。[DOI：10.1063/1.3408289]
Hydration structures at biomolecular surfaces are essential for understanding the mechanisms of the various biofunctions and stability of biomolecules. Here, we demonstrate the measurement of local hydration structures using an atomic force microscopy system equipped with a low-noise deflection sensor. We applied this method to the analysis of the muscovite mica/water interface and succeeded in visualizing a hydration structure that is site-specific on a crystal. Furthermore, at the biomolecule/buffer solution interface, we found surface hydration layers that are more packed than those at the muscovite mica/water interface. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3408289]