The origin of the neoplastic stromal cell in giant cell tumor of bone
The origin of the neoplastic stromal cell in giant cell tumor of bone
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DOI：
10.1053/s0046-8177(03)00413-1
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发表时间：
2003-10-01
期刊：
影响因子：
3.3
通讯作者：
Kaiser, E
中科院分区：
文献类型：
--
作者：
Wülling, M;Delling, G;Kaiser, E
成纤维细胞样基质细胞是骨巨细胞瘤的重要组成部分，在体内和培养的细胞样本中均可观察到。尽管它们被认为触发了GCT的癌症过程，但GCT基质细胞的组织发生机制却鲜为人知。已知间充质干细胞(MSCs)可以分化为成骨细胞。有证据表明，GCT基质细胞也可以发育为成骨细胞。通过两种不同的实验室方法寻找MSCs和GCT基质细胞之间的联系。首先，免疫组织学分析显示，在GCT基质细胞中发现了一些由SH2、SH3、SH4抗体和CD166抗原检测到的相同标记物，与最初的MSCs在成骨细胞分化的第一个发育阶段中发现的相同。这些免疫组织学结果可以通过逆转录聚合酶链式反应来证实。其次，通过形态和谱系特异性染色的细胞分化提供了证据，不仅成骨细胞，而且软骨母细胞和脂肪细胞，都可以从基质细胞培养出来。这种双重方法表明GCT基质细胞可以起源于MSCs。(C)2003 Elsevier Inc.保留所有权利。
Fibroblastlike stromal cells, which are always present as a component of giant cell tumor of bone (GCT), can be observed in both in vivo and cultured cell samples. Although they are assumed to trigger the cancer process in GCT, the histogenesis of GCT stromal cells is poorly understood. It is known that mesenchymal stem cells (MSCs) can develop to osteoblasts. Evidence has been presented that GCT stromal cells can also develop to osteoblasts. A connection between MSCs and GCT stromal cells was sought by using 2 different laboratory approaches. First, immunohistological analyses revealed that some of the same markers, detected by the SH2, SH3, and SH4 antibodies and the CD166 antigen, were found in GCT stromal cells as in the first developmental stages of osteoblast differentiation from the initial MSCs. These immunohistological findings could be confirmed by reverse transcriptase polymerase chain reaction. Second, cellular differentiation by morphology and lineage-specific staining offered evidence that not only osteoblasts, but also chondroblasts and adipocytes, could be cultured from stromal cells. The presented double approach indicates that GCT stromal cells can originate from MSCs. (C) 2003 Elsevier Inc. All rights reserved.