Local effects of amino acid substitutions on the active site region of lysozyme: a comparison of physical and immunological results.
Local effects of amino acid substitutions on the active site region of lysozyme: a comparison of physical and immunological results.
复制标题
氨基酸取代对溶菌酶活性位点区域的局部影响：物理和免疫学结果的比较。
DOI：
10.1021/bi00300a031
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Wilson,AC
中科院分区：
文献类型：
--
作者：
Hornbeck,PV;Wilson,AC
Materials and MethodsChitotriose (ie, tri-TV-acetylglucosamine) was produced by partial acid hydrolysis of chitinand linear gradient elution from water to 60% ethanol on a charcoal-Celite column, 6 cm X 70 cm (Rupley, 1964). The peaks from the charcoal-Celite column that were enriched for the trimer were further sepa-rated on a Bio-Gel P-2 column. The trimeric fraction was shown to be pure with descending paper chromatography in ethyl acetate-pyridine-H20 (5: 3: 2). Antigens. Chicken lysozyme c (Sigma grade 1, lot L-6875) was further purified accordingto methods previously described (Prager & Wilson, 1971a). Bobwhite quail, California quail, turkey, Japanese quail, ring-necked pheasant, duck II, and chachalaca lysozymes were supplied by E. M. Prager and I. M. Ibrahimi of this laboratory. Their purifications and characterizations are described elsewhere (Ibrahimi et al.,
