Clonal expansion and cell dispersion in the developing mouse retina.
Clonal expansion and cell dispersion in the developing mouse retina.
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发育中的小鼠视网膜中的克隆扩增和细胞分散。
DOI：
10.1046/j.1460-9568.1999.00712.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Tan,SS
中科院分区：
文献类型：
--
作者：
Reese,BE;Necessary,BD;Tam,PP;Faulkner-Jones,B;Tan,SS
本研究使用了两种不同的方法标记视泡阶段的前体细胞，以检测发育中的视网膜内克隆性扩张和细胞分散的模式。X失活转基因小鼠和表达LacZ报告基因的嵌合小鼠在发育过程中和成年后被检测，以研究已知身份的增殖神经上皮细胞和有丝分裂后视网膜细胞的径向和切向扩散。嵌合视网膜被用来测量切向分散距离，而转基因视网膜被用来评估单个视网膜神经元群体的切向分散的频率。切向弥散是特定视网膜细胞类型的普遍特征，与其他细胞严格的径向弥散形成对比。切向弥散是一种相对较短的距离现象，锥体细胞、水平细胞、无长突细胞和神经节细胞具有明显的分散距离特征。胚胎和出生后的视网膜显示，这些不同类型的细胞在不同的时间发生切线分散，与它们的分化时间有关，而不是与它们的神经发生时期有关。这些发育结果排除了切向弥散是由于较晚出生的细胞增殖所产生的被动移位，或由于分裂兄弟姐妹的横向弥散所致的可能性；相反，它们与切向弥散在建立有序的视网膜马赛克空间分布中起作用的假设是一致的。
The present study has used two different approaches for labelling progenitor cells at the optic vesicle stage in order to examine patterns of clonal expansion and cellular dispersion within the developing retina. X‐inactivation transgenic mice and chimeric mice expressing the lacZ reporter transgene were examined during development and in adulthood to study the radial and tangential dispersion of proliferating neuroepithelial cells and postmitotic retinal cells of known identities. Chimeric retinas were used to measure tangential dispersion distances, while transgenic retinas were used to assess the frequency of tangential dispersion for individual populations of retinal neurons. Tangential dispersion is shown to be a universal feature of particular retinal cell types, being contrasted with the strictly radial dispersion of other cells. Tangential dispersion is a relatively short‐distance phenomenon, with distinct dispersion distances characteristic for cone, horizontal, amacrine and ganglion cells. Embryonic and postnatal retinas show that tangential dispersion occurs at different times for these distinct cell types, associated with their times of differentiation rather than their neurogenetic periods. These developmental results rule out the possibility that tangential dispersion is due to a passive displacement produced by the proliferation of later‐born cells, or to the lateral dispersion of a dividing sibling; rather, they are consistent with the hypothesis that tangential dispersion plays a role in the establishment of the orderly spatial distribution of retinal mosaics.