Ethylene receptor transcript accumulation patterns during flower senescence in Oncidium 'Gower Ramsey' as affected by exogenous ethylene and pollinia cap dislodgment
Ethylene receptor transcript accumulation patterns during flower senescence in Oncidium 'Gower Ramsey' as affected by exogenous ethylene and pollinia cap dislodgment
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DOI：
10.1016/j.postharvbio.2006.12.012
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发表时间：
2007-05-01
影响因子：
7
通讯作者：
Do, Yi-Yin
中科院分区：
文献类型：
--
作者：
Huang, Wei-Fen;Huang, Pung-Ling;Do, Yi-Yin
克隆了文心兰（Oncidium）的一个乙烯响应传感器（ERS）型乙烯受体OgERS 1的cDNA，并对其推导的氨基酸序列进行了结构分析。乙烯受体同源物的氨基酸序列的系统发育分析表明，OgERS 1是密切相关的其他成员的乙烯受体从单子叶植物。OgERS1在根和花蕾中表达丰富，在假鳞茎、叶和完全开放的花中表达较少。OgERS1 mRNA在完全开放的花朵自然衰老过程中的表达量随剪后天数的增加而增加，在开花的第5天达到最大值。OgERS1 mRNA的积累在乙烯浓度突然升高、花朵开始萎蔫的当天开始下降。OgERS1 mRNA的表达量在去花粉帽文心兰花中表现出先增加后减少的趋势，在花衰老的后期，在未脱落的完全开放的花，和整体表达水平下降，在整个衰老期间，涉及到未脱落的花。我们的研究结果表明，乙烯受体OgERS1在不同器官中的表达存在差异，花粉帽的脱落减轻了外源乙烯处理引发的乙烯受体mRNA水平。(c)2006 Elsevier B.V.保留所有权利。
The cDNA encoding a putative ethylene response sensor (ERS) type ethylene receptor OgERS1 of Oncidium was cloned and the deduced amino acid sequence of OgERS1 was structurally characterized. Phylogenetic analysis of amino acid sequences of ethylene receptor homologues indicated that OgERS1 was closely related to other members of ethylene receptors from monocotyledonous plants. OgERS1 was expressed abundantly in roots and flower buds, and a lesser extent in pseudobulbs, leaves, and fully opened flowers. OgERS1 mRNA levels in fully opened flowers during the natural senescence increased with days after cutting and reached the maximum in the fifth day of bloom. The accumulation of OgERS1 mRNA began to decrease at the day when ethylene raised abruptly and the flowers started to wilt. The expression of OgERS1 mRNA in pollinia de-capped Oncidium flowers showed an increasing and then decreasing trend toward the late stage of flower senescence as in un-dislodged fully opened flowers, and the overall expression level was decreased throughout the senescence period referred to un-dislodged flowers. Our results concluded that the expression of ethylene receptor OgERS1 is differentially expressed in different organs and the pollinia cap dislodgment alleviated the mRNA levels of ethylene receptor initiated by exogenous ethylene treatments. (c) 2006 Elsevier B.V. All rights reserved.