Preparation and characterization of curdlan with unique single-helical conformation and its assembly with Congo Red.
Preparation and characterization of curdlan with unique single-helical conformation and its assembly with Congo Red.
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DOI：
10.1016/j.carbpol.2021.117985
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发表时间：
2021-07
影响因子：
11.2
通讯作者：
Xiaoshuang Yan;Biaolan Liu;Geying Ru;Jiwen Feng
中科院分区：
文献类型：
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作者：
Xiaoshuang Yan;Biaolan Liu;Geying Ru;Jiwen Feng
由于缺乏具有独特特定构象的表征（即，单螺旋或三螺旋）。在这项研究中，在35 - 50 °C的稀NaOH溶液中产生单螺旋凝胶多糖，并使用NMR，SAXS和GPC进行表征。利用核磁共振技术研究了分子从卷曲到单螺旋的构象转变以及分子内氢键相互作用。发现上述两种类型的凝胶多糖以非常不同的方式与刚果红相互作用。单螺旋凝胶多糖可以包裹刚果红形成稳定的超分子染料组装体，这通过凝胶多糖的H3与刚果红的苯基之间的最短距离以及刚果红和凝胶多糖的相同自扩散系数来证明。相比之下，无规卷曲凝胶多糖与刚果红的相互作用较弱，不能包裹它。
Elucidating the structure-activity relationship of curdlan is hampered by a lack of characterization with unique specific conformations (i.e., single- or triple-helix). In this study, single-helical curdlan is generated in dilute NaOH solutions at 35−50 °C, and characterized with NMR, SAXS, and GPC. The conformational transition from coil to single-helix and the intramolecular hydrogen bond interaction are explored using NMR. It is found that the two aforementioned types of curdlan interact with Congo Red in very different ways. Single-helical curdlan can encapsulate Congo Red to form a stable, supramolecular dye assembly, which is demonstrated by the shortest distance between the H3 of curdlan and the phenyl groups of Congo Red, and also the same self-diffusion coefficients of Congo Red and curdlan. In contrast, random-coil curdlan interacts weakly with Congo Red and cannot enwrap it. This study offers insight into the specific structure-activity relationship of beta-(1,3)-glucans.