Macronuclear transformation with specific DNA fragments controls the content of the new macronuclear genome in Paramecium tetraurelia.
Macronuclear transformation with specific DNA fragments controls the content of the new macronuclear genome in Paramecium tetraurelia.
复制标题
使用特定 DNA 片段的大核转化控制草履虫中新大核基因组的内容。
DOI：
10.1128/mcb.11.2.1133-1137.1991
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发表时间：
1991
影响因子：
5.3
通讯作者：
Forney,J
中科院分区：
文献类型：
--
作者：
You,Y;Aufderheide,K;Morand,J;Rodkey,K;Forney,J
先前分离的称为d48的突变细胞系在微核基因组中含有A表面抗原基因的完整拷贝，但该基因未并入大核。先前的实验已经表明，在野生型大核中产生的细胞质因子可以拯救突变体。最近，S.小泉和S.小林（Mol. Cell. 9：4398-4401，1989）观察到将含有A基因的质粒注射到d48大核中拯救了自花受精后的细胞系。这里表明，仅含有A基因编码区的一部分的8.8-kbEcoRI片段足以拯救d48。其他A基因片段不能拯救突变体，表明这种效应依赖于特定的草履虫DNA序列。拯救导致大核中野生型DNA限制性模式的恢复。这些结果是一致的模型，其中大核A基因座通常使一个额外的基因产物，所需的正确处理的微核拷贝的A基因。
A previously isolated mutant cell line called d48 contains a complete copy of the A surface antigen gene in the micronuclear genome, but the gene is not incorporated into the macronucleus. Previous experiments have shown that a cytoplasmic factor made in the wild-type macronucleus can rescue the mutant. Recently, S. Koizumi and S. Kobayashi (Mol. Cell. Biol. 9:4398-4401, 1989) observed that injection of a plasmid containing the A gene into the d48 macronucleus rescued the cell line after autogamy. It is shown here that an 8.8-kbEcoRI fragment containing only a portion of the A gene coding region is sufficient for the rescue of d48. The inability of other A gene fragments to rescue the mutant shows that this effect is dependent upon specificParameciumDNA sequences. Rescue results in restoration of the wild-type DNA restriction pattern in the macronucleus. These results are consistent with a model in which the macronuclear A locus normally makes an additional gene product that is required for correct processing of the micronuclear copy of the A gene.