Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells

Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells
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DOI：
10.1007/978-1-4939-7799-4_4
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发表时间：
2018-01-01
期刊：
ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
通讯作者：
Dani, Christian
Dani, Christian
中科院分区：
其他
文献类型：
--
作者：
Hafner, Anne-Laure;Mohsen-Kanson, Tala;Dani, Christian

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在本章中，我们描述了一种高速率诱导来自人诱导多能干细胞（hiPSCs）的棕色/棕色样脂肪细胞祖细胞（BAPs）分化的方法。我们还描述了维持hipsc和获得hiPSC-BAPs的培养条件。这种新的培养系统提供了人类棕色脂肪细胞的无限来源，并为研究调节人类BAPs的产生和募集的事件提供了独特的手段。
In this chapter, we describe a protocol to induce at a high rate the differentiation of brown/brown like adipocyte progenitors (BAPs) derived from human induced pluripotent stem cells (hiPSCs). We also describe culture conditions to maintain hiPSCs and to derive hiPSC-BAPs.This novel culture system provides an unlimited source of human brown adipocytes and a unique means for studying events regulating the generation and recruitment of human BAPs.