Taura syndrome of marine penaeid shrimp: Characterization of the viral agent

Taura syndrome of marine penaeid shrimp: Characterization of the viral agent
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DOI:
10.1099/0022-1317-78-2-313
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发表时间:
1997-02-01
影响因子:
3.8
通讯作者:
Lightner, DV
Lightner, DV
中科院分区:
医学3区
文献类型:
--
作者:
Bonami, JR;Hasson, KW;Lightner, DV

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Taura综合征(TS)的病原体在1994年被确认为病毒性质,并暂时归类为属于小核糖核酸病毒科或野大卫病毒科。这里报道的工作导致了这种新的对虾病毒的更明确的分类。该病毒位于感染的对虾表皮上皮细胞的细胞质内,是一个31至32 nm的二十面体颗粒,浮力密度为1。338+/-0。001 μ g/ml。三个主要的(55,40和24 kDa)和一个次要的(58 kDa)多肽构成其蛋白质衣壳。它的基因组含有一个ssRNA分子,在3'端聚腺苷酸化,长度约为9 kb。基于这些特征,我们认为TS病毒应被纳入小核糖核酸病毒科。厄瓜多尔和夏威夷的TS病毒分离株在生物物理、生物化学和生物学特性上完全相同,应视为同一病毒。
The causative agent of Taura syndrome (TS) was recognized in 1994 to be viral in nature and tentatively classified as belonging to either the family Picornaviridae or Nodaviridae. The work reported here has led to a more definitive classification of this new penaeid virus. Located within the cytoplasm of infected cuticular epithelial cells of penaeid shrimp, the virus is a 31 to 32 nm icosahedral particle with a buoyant density of 1 . 338+/-0 . 001 g/ml. Three major (55, 40 and 24 kDa) and one minor (58 kDa) polypeptides constitute its proteinic capsid. Its genome contains a single molecule of ssRNA, which is polyadenylated at the 3' end and approximately 9 kb in length. Based on these characteristics, we believe that TS virus should be included in the family Picornaviridae. Ecuadorian and Hawaiian TS virus isolates were found to be identical in their biophysical, biochemical and biological characteristics, and should be considered as the same virus.