ENHANCED DNA-BINDING ACTIVITY OF A STAT3-RELATED PROTEIN IN CELLS TRANSFORMED BY THE SRC ONCOPROTEIN
ENHANCED DNA-BINDING ACTIVITY OF A STAT3-RELATED PROTEIN IN CELLS TRANSFORMED BY THE SRC ONCOPROTEIN
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DOI：
10.1126/science.7541555
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发表时间：
1995-07-07
期刊：
影响因子：
56.9
通讯作者：
JOVE, R
中科院分区：
文献类型：
--
作者：
YU, CL;MEYER, DJ;JOVE, R
细胞因子和生长因子诱导直接激活基因表达的信号转导子和转录激活子（STAT）的酪氨酸磷酸化。检测Src癌基因酪氨酸激酶稳定转化的细胞的STAT蛋白活化。电泳迁移率，DNA结合特异性和抗原性的测定表明，Stat3或密切相关的STAT家族成员组成型激活的Src癌蛋白。这种DNA结合活性的诱导伴随着Stat3的酪氨酸磷酸化，并与Src转化相关。这些发现表明Src可以激活STAT信号通路，并提高了Stat3通过Src参与肿瘤发生的可能性。
Cytokines and growth factors induce tyrosine phosphorylation of signal transducers and activators of transcription (STATs) that directly activate gene expression. Cells stably transformed by the Src oncogene tyrosine kinase were examined for STAT protein activation. Assays of electrophoretic mobility, DNA-binding specificity, and antigenicity indicated that Stat3 or a closely related STAT family member was constitutively activated by the Src oncoprotein. Induction of this DNA-binding activity was accompanied by tyrosine phosphorylation of Stat3 and correlated with Src transformation. These findings demonstrate that Src can activate STAT signaling pathways and raise the possibility that Stat3 contributes to oncogenesis by Src.