Acetate supplementation increases brain phosphocreatine and reduces AMP levels with no effect on mitochondrial biogenesis.
Acetate supplementation increases brain phosphocreatine and reduces AMP levels with no effect on mitochondrial biogenesis.
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DOI：
10.1016/j.neuint.2013.01.004
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发表时间：
2013-02
影响因子：
4.2
通讯作者：
Rosenberger TA
中科院分区：
文献类型：
--
作者：
Bhatt DP;Houdek HM;Watt JA;Rosenberger TA
大鼠补充醋酸盐可增加血浆醋酸盐和脑乙酰辅酶A水平。虽然醋酸盐被用作研究神经胶质细胞能量代谢的标志物，但醋酸盐补充对正常大脑能量储存的影响尚未量化。为了确定乙酰辅酶A水平的增加对脑能量代谢的影响，我们测量了脑核苷酸、磷酸原和糖原水平，并定量了补充乙酸盐的大鼠的心磷脂含量和线粒体数量。通过经口管饲法（6 g/Kg体重）用三乙酸甘油酯（GTA）诱导乙酸盐补充。用于生化分析的大鼠在处理后2和4小时使用头部聚焦微波辐射实施安乐死，以立即停止代谢。我们发现，与对照组相比，醋酸盐未改变脑ATP、ADP、NAD、GTP水平或能荷比[ECR，（ATP + ½ ADP）/（ATP + ADP + AMP）]。然而，治疗4小时后，脑磷酸肌酸水平显著升高，伴随AMP水平降低，糖原水平无变化。在平行研究中，大鼠与GTA治疗28天，我们发现，乙酸并没有改变脑糖原和线粒体生物合成测定脑心磷脂含量，心磷脂的脂肪酸组成，并使用定量超微结构分析，以确定海马CA 3神经元的线粒体密度/单位面积的细胞质。总的来说，这些数据表明，通过补充乙酸盐增加脑乙酰辅酶A水平确实增加了脑能量储存，但对脑糖原和神经元线粒体生物合成没有影响。
Acetate supplementation in rats increases plasma acetate and brain acetyl-CoA levels. Although acetate is used as a marker to study glial energy metabolism, the effect that acetate supplementation has on normal brain energy stores has not been quantified. To determine the effect(s) that an increase in acetyl-CoA levels has on brain energy metabolism, we measured brain nucleotide, phosphagen and glycogen levels, and quantified cardiolipin content and mitochondrial number in rats subjected to acetate supplementation. Acetate supplementation was induced with glyceryl triacetate (GTA) by oral gavage (6 g/Kg body weight). Rats used for biochemical analysis were euthanized using head-focused microwave irradiation at 2, and 4 hr following treatment to immediately stop metabolism. We found that acetate did not alter brain ATP, ADP, NAD, GTP levels, or the energy charge ratio [ECR, (ATP + ½ ADP) / (ATP + ADP + AMP)] when compared to controls. However, after 4 hr of treatment brain phosphocreatine levels were significantly elevated with a concomitant reduction in AMP levels with no change in glycogen levels. In parallel studies where rats were treated with GTA for 28 days, we found that acetate did not alter brain glycogen and mitochondrial biogenesis as determined by measuring brain cardiolipin content, the fatty acid composition of cardiolipin and using quantitative ultra-structural analysis to determine mitochondrial density/unit area of cytoplasm in hippocampal CA3 neurons. Collectively, these data suggest that an increase in brain acetyl-CoA levels by acetate supplementation does increase brain energy stores however it has no effect on brain glycogen and neuronal mitochondrial biogenesis.
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影响因子：
9.3
作者：
Brissette CA;Houdek HM;Floden AM;Rosenberger TA
通讯作者：
Rosenberger TA
影响因子：
2.9
作者：
HAMMER, DF;UNVERFERTH, DV;ALTSCHULD, RA
通讯作者：
ALTSCHULD, RA
影响因子：
4.8
作者：
Herzog, Raimund I.;Chan, Owen;Sherwin, Robert S.
通讯作者：
Sherwin, Robert S.
影响因子：
2.9
作者：
Gupta, RC;Dettbarn, WD
通讯作者：
Dettbarn, WD
影响因子：
4.2
作者：
Arun, Peethambaran;Ariyannur, Prasanth S.;Namboodiri, Aryan M. A.
通讯作者：
Namboodiri, Aryan M. A.