Ciliary responsiveness in allergic and nonallergic airways.
Ciliary responsiveness in allergic and nonallergic airways.
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过敏性和非过敏性气道的纤毛反应性。
DOI：
10.1152/jappl.1986.60.6.1967
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Abraham,WM
中科院分区：
文献类型：
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作者：
Wanner,A;Sielczak,M;Mella,JF;Abraham,WM
过敏性哮喘与呼吸道(平滑肌)对几种化学过敏介质的高反应性有关；然而，目前尚不清楚这是否伴随着粘液纤毛高反应性。因此，本研究的目的是确定与非过敏性绵羊相比，过敏性绵羊的呼吸道纤毛活动是否对前列腺素E1(PGE1)、前列腺素E2(PGE2)和白三烯D4(LTD4)表现出夸大的反应，LTD4的作用是直接的或涉及花生四烯酸代谢环氧合酶产物的产生。用显微镜技术在灌注室中测量纤毛搏动频率(CBF)，用从“过敏性”绵羊(对吸入的特定抗原有阳性皮肤反应和既往的支气管痉挛反应)和“非过敏性”(无皮肤反应阴性，无吸入抗原刺激)绵羊的气管上皮细胞中获得细胞。平均基线CBF在不同组之间没有差异；PGE1、PGE2和LTD4诱导CBF呈剂量依赖性增加，这些增加在过敏和非过敏绵羊中没有差异。在最高激动剂浓度时，CBF较基线平均增加13%~16%(P<0.05)。LTD4的纤毛刺激作用可被硫多肽白三烯拮抗剂fpl-55712和环氧合酶抑制剂消炎痛显著减弱。这些结果表明，过敏性绵羊对选定的化学过敏介质不表现出纤毛高反应性，LTD4的纤毛刺激作用依赖于环氧合酶的激活，可能还依赖于前列腺素的产生。
Allergic asthma is associated with airway (smooth muscle) hyperresponsiveness to several chemical mediators of anaphylaxis; however, it is not known whether this is accompanied by mucociliary hyperresponsiveness. The purpose of this study was therefore to determine if airway ciliary activity, a component function of mucociliary clearance, exhibits exaggerated responses to prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), and leukotriene D4 (LTD4) in allergic sheep when compared with nonallergic sheep, and the effects of LTD4 are direct or involve the generation of cyclooxygenase products of arachidonate metabolism. Ciliary beat frequency (CBF) was measured in a perfusion chamber with a microscopic technique using tracheal epithelial cells obtained from brushing of “allergic” (positive cutaneous reaction and previous bronchospastic response to inhaled specific antigen) and “nonallergic” (negative cutaneous reaction, no previous inhalation challenge with antigen) sheep. Mean base-line CBF was not different among the groups; PGE1, PGE2, and LTD4 induced dose-dependent increases in CBF, and these increases were not different in allergic and nonallergic sheep. At the highest agonist concentration the mean increase in CBF from base line varied between 13 and 16% (P less than 0.05). The ciliostimulatory effect of LTD4 was significantly blunted by both the sulfidopeptide leukotriene antagonist FPL-55712 and the cyclooxygenase inhibitor indomethacin. These results suggest that allergic sheep fail to exhibit ciliary hyperresponsiveness to selected chemical mediators of anaphylaxis and the ciliostimulatory effect of LTD4 depends on the activation of cyclooxygenase and possibly the generation of prostaglandins.