Proteome analysis of whole saliva: A new tool for rheumatic diseases - the example of Sjogren's syndrome
Proteome analysis of whole saliva: A new tool for rheumatic diseases - the example of Sjogren's syndrome
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DOI：
10.1002/pmic.200600783
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发表时间：
2007-05-01
期刊：
影响因子：
3.4
通讯作者：
Lucacchini, Antonio
中科院分区：
文献类型：
--
作者：
Giusti, Laura;Baldini, Chiara;Lucacchini, Antonio
干燥综合征（pSS）是一种直接影响唾液腺的全身性疾病，因此预计会影响人类全唾液（WS）液的成分。本研究的目的是使用蛋白质组学方法来表征 pSS 患者的 WS 蛋白，以评估检查结缔组织疾病中唾液蛋白谱整体变化的有效程序。使用 2-DE 技术分析了 pSS 患者和健康志愿者中表达的 WS 蛋白。与对照组相比，pSS 患者的 WS 蛋白模式发生了改变，一些典型的唾液蛋白减少了。特别是，观察到碳酸酐酶VI的显着改变。此外，对 pSS 患者的 WS 蛋白谱与对照组的 WS 蛋白谱进行比较，发现了一组差异表达的蛋白质。这些蛋白质与急性和慢性炎症有关，而其他一些蛋白质则与氧化应激损伤有关。这些发现与 pSS 的全身免疫炎症方面一致，并为系统性搜索 pSS 的诊断生物标志物和治疗干预靶标提供了可能性。
Sjogren's syndrome (pSS) is a systemic disease that affects salivary glands directly, and is therefore expected to influence the composition of human whole saliva (WS) fluid. The aim of this study was to characterize the WS proteins of pSS patients using a proteomic approach to assess a valid procedure to examine the global changes of the salivary protein profiles in connective tissue disorders. The WS proteins expressed in patients affected by pSS and healthy volunteers were analyzed using the 2-DE technique. The WS protein pattern was altered in pSS patients compared to controls, with a decrease in some of the typical salivary proteins. Particularly, a remarkable alteration of carbonic anhydrase VI was observed. Moreover, a comparison of WS protein profile of pSS patients with the one obtained from controls revealed a set of differentially expressed proteins. These proteins were related to acute and chronic inflammation while some others were involved in oxidative stress injury. These findings are in line with the systemic immunoinflammatory aspects of pSS and open the possibility for a systematic search of diagnostic biomarkers and targets for therapeutic intervention in pSS.