Antigenic variants of influenza A virus, PR8 strain. I. Their development during serial passage in the lungs of partially immune mice.
Antigenic variants of influenza A virus, PR8 strain. I. Their development during serial passage in the lungs of partially immune mice.
复制标题
流感病毒的抗原变体Pr8菌株。 I.它们在部分免疫小鼠的肺中连续通过期间发育。
DOI：
10.1084/jem.101.6.627
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发表时间：
1955-06-01
影响因子：
15.3
通讯作者：
HAMBRE, D
中科院分区：
文献类型：
--
作者：
GERBER, P;LOOSLI, C G;HAMBRE, D
通过在用同源试剂免疫的小鼠的肺中连续传代17次，产生了小鼠适应的PR 8甲型流感病毒的抗原性不同的毒株。比较血清学试验表明，变异株与亲本株具有相同的抗原成分，但优势抗原不同。通过抗体吸收，表明变体的“新”抗原组分在第8代之前已经以少量存在，此后随着在接种小鼠中的继续传代而显著增加。用PR 8-S或T21病毒接种并具有相当抗体滴度的小鼠组在用同源毒株进行辅助性攻击后在肺中未显示病毒生长。另一方面，在异源空气传播攻击后，未发生死亡，但病毒在两组接种小鼠的肺中生长。在接种亲本菌株并用PR 8-T21病毒攻毒的小鼠的肺中发生几乎不受限制的病毒增殖，导致广泛的实变。用变异株接种并用PR 8-S病毒攻击的小鼠的肺中生长的病毒较少。在这些动物中，仅观察到肺部病毒生长引起变化的显微镜证据。PR 8甲型流感病毒在免疫动物中的成功连续传代取决于具有一致低H. I.如在尾血上测定的抗体滴度，以及鼻内滴注足够的病毒以有利于与存在的抗体最不相关的那些病毒颗粒的存活。流行病学的影响，这些意见进行了简要讨论。
Antigenically different strains of mouse-adapted PR8 influenza A virus have been produced by 17 serial passages of the virus in the lungs of mice immunized with the homologous agent. Comparative serological tests show that the variant strains share antigenic components with the parent strain but the dominant antigen is different. By means of antibody absorption it was shown that the "new" antigenic component of the variant was already present in minor amounts up to the eighth passage and thereafter gained prominence with continued passage in vaccinated mice. Groups of mice vaccinated with either the PR8-S or T21 virus and having comparable antibody titers showed no growth of virus in the lungs following aid-borne challenge with homologous strains. On the other hand, following heterologous air-borne challenge no deaths occurred, but virus grew in the lungs of both groups of vaccinated mice. Almost unrestricted virus multiplication took place in the lungs of mice vaccinated with the parent strain and challenged with the PR8-T21 virus which resulted in extensive consolidation. Less virus grew in the lungs of the mice vaccinated with the variant strains and challenged with the PR8-S virus. In these animals only microscopic evidence of changes due to virus growth in the lungs was observed. The successful serial passage of PR8 influenza A virus in immunized animals was dependent on the initial selection of mice with uniformly low H.I. antibody titers as determined on tail blood, and the intranasal instillation of sufficient virus to favor the survival of those virus particles least related to the antibodies present. The epidemiological implications of these observations are discussed briefly.