Analysis of functional genomes from metagenomes: Revealing the accelerated electron transfer in microbial fuel cell with rhamnolipid addition
Analysis of functional genomes from metagenomes: Revealing the accelerated electron transfer in microbial fuel cell with rhamnolipid addition
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宏基因组的功能基因组分析：揭示添加鼠李糖脂的微生物燃料电池中电子转移的加速
DOI：
10.1016/j.bioelechem.2017.08.010
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发表时间：
2018-02-01
影响因子：
5
通讯作者：
Yu, Hang
中科院分区：
文献类型：
--
作者：
Zhang, Yunshu;Jiang, Junqiu;Yu, Hang
胞外电子传递是微生物燃料电池（MFC）的主要产电过程。我们前期的研究已经证明了鼠李糖脂的阳极吸附产生了Frumkin效应，这种效应增强了阳极微生物的附着，加速了阳极电子传递。本研究从基因水平上深入研究了鼠李糖脂对阳极生物膜宏基因组功能基因的影响，并从基因水平上解释其作用机制。结果表明，各优势属的功能基因组成和分布不同。信号转导机制的类别是占主导地位的功能类别外泌电，其相对丰度的宏基因组显着增加从4.56%至5.86%，从鼠李糖脂添加。此外，代谢途径和电子流分析显示，在存在鼠李糖脂的情况下，电子流倾向于选择直接电子转移，并导致库仑效率从19.10 +/- 0.79%增加到2739 +/-1.07%。(C)2017爱思唯尔B. V.保留所有权利。
Extracellular electron transfer is the predominant electricity generation process in microbial fuel cells (MFCs). Our pervious study have proved that the anodic adsorption of rhamnolipid resulted in the Frumkin effect, which enhanced anodic microorganism attachment and accelerated anodic electron transfer. In this study, an in-depth research on the influence of rhamnolipid on functional genes of anodic biofilms metagenomes was carried out to explain its mechanism at the gene level. The result showed that the composition and distribution of functional genes in each dominant genus were different. The category of signal transduction mechanisms was the dominant function category in exoelectrogens, and its relative abundance in the metagenome significantly increased from 4.56 to 5.86% from rhamnolipid addition. Additionally, the metabolic pathway and electron flow analysis revealed that electron flows tend to choose direct electron transfer in the presence of rhamnolipids, and resulting in the increase of Coulombic efficiency from 19.10 +/- 0.79% to 2739 +/- 1.07%. (C) 2017 Elsevier B.V. All rights reserved.