Characterization of novel natural killer (NK)-cell and γδ T-cell lines established from primary lesions of nasal T/NK-cell lymphomas associated with the Epstein-Barr virus
Characterization of novel natural killer (NK)-cell and γδ T-cell lines established from primary lesions of nasal T/NK-cell lymphomas associated with the Epstein-Barr virus
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DOI：
10.1182/blood.v97.3.708
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发表时间：
2001-02-01
期刊：
影响因子：
20.3
通讯作者：
Shimizu, N
中科院分区：
文献类型：
--
作者：
Nagata, H;Konno, A;Shimizu, N
鼻T/自然杀伤（NK）细胞淋巴瘤的研究因其易引起坏死而受到阻碍，因此，建立鼻NK细胞淋巴瘤细胞系具有重要价值。本研究尝试从该肿瘤原发灶建立细胞系，并通过高剂量重组白细胞介素2的方法成功获得了2株新的EB病毒（EBV）阳性细胞系SNK-6和SNT-8。流式细胞术显示SNK-6具有NK细胞表型，CD 3（-）CD 4（-）CD 8（-）CD 19（-）CD 56（+）T细胞受体（TCR）α/β（-）TCR γ/δ（-），而SNT-8是CD 3（+）CD 4（-）CD 8（-）CD 19（-）CD 56（+）TCR α/β（-）TCR γ/δ（+），这些与其原始肿瘤的免疫表型一致，并且细胞系具有与其原始肿瘤中的单克隆EBV克隆相同的单克隆EBV克隆，因此，细胞系从形成原发性病变的细胞发育而来。基因型分析显示SNK-6具有未重排的TCR和免疫球蛋白重链基因，支持SNK-6是NK细胞谱系的结论。另一方面，SNT-8具有重排的TCR β、γ和δ链基因，并且结合其表型，证明SNT-8是γ δ T细胞系。本文首次从鼻T/NK细胞淋巴瘤的原发灶中建立了细胞系，结果表明，鼻T/NK细胞淋巴瘤至少存在NK细胞和γ δ T细胞两个谱系，而且由于两种类型的病例具有共同的临床和组织病理学特征，提示这两个谱系的鼻T/NK细胞淋巴瘤可能属于同一临床病理实体。(C)2001年，美国血液学会。
Studies on nasal T/natural killer (NK)-cell lymphoma have been hampered by its tendency to cause necrosis, Thus, the establishment of cell lines of this neoplasm would seem to be valuable. This study attempted to establish cell lines from primary lesions of this tumor, and successfully obtained 2 novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high-dose recombinant interleukin 2, Flow cytometry showed that SNK-6 had an NK-cell phenotype,CD3(-)CD4(-)CD8(-)CD19(-)CD56(+) T-cell receptor (TCR) alpha/beta (-) TCR gamma/delta (-,) whereas SNT-8 was CD3(+)CD4(-)CD8(-)CD19(-)CD56(+) TCR alpha/beta (-) TCR gamma/delta (+), These were consistent with immunophenotypes of their original tumors, and the cell lines had monoclonal EBV clones identical to ones in their original tumors, Thus, the cell lines developed from cells forming the primary lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6 was of NK-cell lineage, On the other hand, SNT-8 had rearranged TCR beta-, gamma-, and delta -chain genes, and together with its phenotype, SNT-8 proved to be a gamma delta T-cell line. This is the first report of the establishment of cell lines from primary lesions of nasal T/NK cell lymphomas, and the results demonstrated that there are at least 2 lineages, NK- and gamma delta T cell, in this neoplasm, Moreover, it has been suggested that nasal T/NK cell lymphomas of these lineages may belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features. (C) 2001 by The American Society of Hematology.