DEVELOPMENT OF CELL AND FIBER LAMINATION IN THE MOUSE SUPERIOR COLLICULUS
DEVELOPMENT OF CELL AND FIBER LAMINATION IN THE MOUSE SUPERIOR COLLICULUS
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DOI：
10.1002/cne.902480308
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发表时间：
1986-06-15
影响因子：
2.5
通讯作者：
SCHNEIDER, GE
中科院分区：
文献类型：
--
作者：
EDWARDS, MA;CAVINESS, VS;SCHNEIDER, GE
采用多种解剖方法，包括氚胸腺自显影法，对小鼠上丘层状组织的出现进行了研究。高尔基浸渍，以及细胞体和纤维的一般染色。神经发生、细胞迁移和神经元早期形态分化的顺序显示出下层和上层之间的不连续性（即深层和中间的“灰色”和“白色”层与浅层“灰色”和“白色”层之间）。这些项目在低级别联赛中按照由内到外的顺序进行，但在高级别联赛中同样的项目在完成这一进程之前开始。因此，下层各层的峰值产生时间从（胚胎日）E11到E13，上层各层的峰值产生时间从E12到E13。用氚化胸腺嘧啶标记监测的细胞迁移密切反映了细胞发生的模式。这在E15上最为明显，当e11标记的细胞群体被划分为浅层和深层（浅层和深层- ss和SP层）时，在中间水平（层间- si）插入e13标记的细胞。E17是细胞迁移基本完成的时间点，后一组细胞继续迁移，并在SS内加入前一组细胞。在E13 ~ E17这段时间内，神经纤维束的积累和神经元早期形态分化的时间进程与神经元到达和三初级层形成的顺序相对应，都趋向于从SP到SS和从SS到SI。因此，SP的横向纤维系统和大多极神经元先于SS的纵向纤维系统和垂直定向神经元发育，而SS的纵向纤维系统和中等多极神经元发育早于SI的纵向纤维和中等多极神经元。相比之下，在E17至P6（出生后），主要径向生长和构造亚分层下的后期分化事件以一种更简单的由内向外的顺序进行。在丘中建立均一化的主要形态发生事件似乎涉及两个基本分支相对独立的组装程序的操作。可能是选择性的细胞-细胞相互作用促进了同时生成的神经元向不同层的传递，以及轴突以一种尊重层边界的方式的部署。
The emergence of laminar organization in the superior colliculus was investigated in the mouse with several anatomical methods, including tritiated-thymidine autoradiography. Golgi impregnation, and general stains for cell bodies and for fibers. The sequence of neurogenesis, cell migration, and early morphological differentiation of neurons was shown to exhibit a discontinuity between the lower and upper divisions (i.e., between the deep and intermediate "gray" and "white" layers and the superficial "gray" and "white" layers). These events proceed in an inside-out order within the lower division, but the same events within the upper division commence in advance of the completion of this progression. Thus, peak generation times for layers of the lower division proceed from (embryonic day) E11 to E13 and for the upper division from E12 to E13. Cell migration, as monitored with tritiated-thymidine labelling, reflects closely the pattern of cytogenesis. This is most clearly evident on E15 when a population of E11-labelled cells is divided into superficial and deep layers (the strata superficiale and profundum-SS and SP) by the interpostion of E13-labelled cells at an intermediate level (stratum intermedium-SI). A contingent of the latter cells continue their migration and join their predecessors within the SS on E17, a time point when cell migrations are largely complete. Paralleling this sequence of arrival of neurons and the formation of three primary layers, both the time course of accumulation of fiber fascicles and the early morphological differentiation of neurons in the interval from E13 to E17 tends to proceed form SP to SS and from SS to SI. Thus, the transverse fiber system and large multipolar neurons of SP develop in advances of the longitudinal fiber system and vertically oriented neurons of SS, which in turn develop precociously with respect to the longitudinal fibers and medium-sized multipolar neurons of SI. In contrast, later events of differentiation that underly a major radial growth and an architectonic sublamination of the primary strata proceed in a simpler inside-out sequence form E17 to (postnatal day) P6. The major morphogenetic events underlying the establishment of statification in the colliculus appear to involve the operation of relatively independent programs of assembly for the two basic subdivisions. It is probable that selective cell-cell interactions contribute to the delivery of concurrently generated neurons to different laminae as well as to the deployment of axons in a manner that respects laminar boundaries.