The Gas2 family protein Pigs is a microtubule +TIP that affects cytoskeleton organisation.
The Gas2 family protein Pigs is a microtubule +TIP that affects cytoskeleton organisation.
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DOI：
10.1242/jcs.176230
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发表时间：
2016-01-01
影响因子：
4
通讯作者：
Röper K
中科院分区：
文献类型：
--
作者：
Girdler GC;Applewhite DA;Perry WM;Rogers SL;Röper K
不同细胞骨架系统之间的协调对许多细胞生物学功能至关重要，包括细胞迁移和有丝分裂，在组织形态发生中也起着重要作用。细胞骨架交联剂或细胞连接剂一类的蛋白质，具有同时与多个细胞骨架系统相互作用的能力，是介导任何协调的主要候选者。其中一类包括Gas2样蛋白，结合保守的钙钙蛋白同源型肌动蛋白结合结构域和预测结合微管（mt）的Gas2结构域。这种结构域组合也存在于谱蛋白中，谱蛋白是一种巨大的细胞连接物，在无脊椎动物和脊椎动物的许多组织中都起着重要作用。在这里，我们分析了单个果蝇gas2样蛋白猪在体外和体内与肌动蛋白和MT细胞骨架相互作用的能力，并说明了复杂的调节相互作用，这些相互作用决定了猪对细胞骨架的定位及其对细胞骨架的影响。摘要：详细分析了果蝇细胞连接子蛋白猪调节其细胞骨架相互作用功能（+TIP跟踪、微管轴结合、肌动蛋白结合和肌动蛋白-微管交联）的能力。
Coordination between different cytoskeletal systems is crucial for many cell biological functions, including cell migration and mitosis, and also plays an important role during tissue morphogenesis. Proteins of the class of cytoskeletal crosslinkers, or cytolinkers, have the ability to interact with more than one cytoskeletal system at a time and are prime candidates to mediate any coordination. One such class comprises the Gas2-like proteins, combining a conserved calponin-homology-type actin-binding domain and a Gas2 domain predicted to bind microtubules (MTs). This domain combination is also found in spectraplakins, huge cytolinkers that play important roles in many tissues in both invertebrates and vertebrates. Here, we dissect the ability of the single Drosophila Gas2-like protein Pigs to interact with both actin and MT cytoskeletons, both in vitro and in vivo, and illustrate complex regulatory interactions that determine the localisation of Pigs to and its effects on the cytoskeleton. Summary: Detailed analysis of the ability of the Drosophila cytolinker protein Pigs to modulate its cytoskeleton-interacting functions (+TIP tracking, microtubule shaft binding, actin binding and actin–microtubule crosslinking).