A novel AT-rich DNA binding protein that combines an HMG I-like DNA binding domain with a putative transcription domain.
A novel AT-rich DNA binding protein that combines an HMG I-like DNA binding domain with a putative transcription domain.
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DOI：
10.1105/tpc.6.1.107
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发表时间：
1994-01
期刊：
影响因子：
--
通讯作者：
G. Tjaden;G. Coruzzi
中科院分区：
文献类型：
--
作者：
G. Tjaden;G. Coruzzi
越来越多的证据表明，富含AT的启动子元件在植物基因转录中发挥作用。对于豌豆叶绿体谷氨酰胺合成酶(GS2)的核基因启动子，从GS2启动子-β-葡萄糖醛酸酶(GUS)融合的5‘端缺失33个核苷酸的AT富含序列(BOX 1)，导致GUS活性下降10倍。用盒1天然元素进行凝胶位移分析，检测到两种不同的复合体。一个复合体与先前报道的来自其他几个植物启动子的富含AT元素的低迁移率复合体有关。使用盒1序列的多聚体分离编码富含AT的DNA结合蛋白(ATBP-1)的cDNA。ATBP-1不是一个高迁移率基团蛋白，但它是一个新的蛋白质，它结合了一个高迁移率基团I/Y类DNA结合域和一个富含谷氨酰胺的假定转录结构域。
There is growing evidence that AT-rich promoter elements play a role in transcription of plant genes. For the promoter of the nuclear gene for chloroplast glutamine synthetase from pea (GS2), the deletion of a 33-bp AT-rich sequence (box 1 native) from the 5' end of a GS2 promoter-beta-glucuronidase (GUS) fusion resulted in a 10-fold reduction in GUS activity. The box 1 native element was used in gel shift analysis and two distinct complexes were detected. One complex is related to the low-mobility complex reported previously for AT-rich elements from several other plant promoters. A multimer of the box 1 sequence was used to isolate a cDNA encoding an AT-rich DNA binding protein (ATBP-1). ATBP-1 is not a high-mobility group protein, but it is a novel protein that combines a high-mobility group I/Y-like DNA binding domain with a glutamine-rich putative transcriptional domain.