Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway
Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway
复制标题
长链非编码RNA FTH1P3通过SP1/NF-kB通路调控食管鳞癌的转移和侵袭。
DOI：
10.1016/j.biopha.2018.07.129
复制
发表时间：
2018-10-01
影响因子：
7.5
通讯作者：
He, Fucheng
中科院分区：
文献类型：
--
作者：
Yang, Lijun;Sun, Kaiyan;He, Fucheng
目的 : : 最新研究表明，长非蛋白编码RNA铁蛋白重链1假基因3（FTH1P3）在肿瘤形成过程中发挥着至关重要的作用。本研究旨在探讨其在食管鳞状细胞癌（ESCC）中的作用。主要方法：采用定量逆转录酶聚合酶链反应（qRT-PCR）检测食管鳞状细胞癌样本和细胞系中FTH1P3、mRNA SP1和NF-kB的表达水平。通过 WST-1 测定、集落形成测定、划痕测定、迁移和侵袭测定评估 FTH1P3 敲低的影响。主要发现：FTH1P3 在 ESCC 组织和细胞中显着上调 (P < 0.001)。 FTH1P3的敲低显着降低了ESCC细胞的增殖、迁移和侵袭能力。沉默FTH1P3可降低EC9706和EC1中特异性蛋白1（Sp1）和NF-kB（p65）的表达。意义：FTH1P3在ESCC肿瘤发生中起着至关重要的作用，可作为ESCC的潜在治疗靶点。
Aims : : Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).Main methods: Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays.Key findings: FTH1P3 was significantly upregulated in ESCC tissues and cells (P < 0.001). Knockdown of FTH1P3 notably decreased the proliferation, migration, and invasion capacity of ESCC cells. Silencing of FTH1P3 decreased the expression of specificity protein 1 (Sp1) and NF-kB (p65) in EC9706 and EC1.Significance: FTH1P3 plays a crucial role in ESCC tumorigenesis, and can be used as a potential therapeutic target for ESCC.