Overexpression of a host factor TOM1 inhibits tomato mosaic virus propagation and suppression of RNA silencing
Overexpression of a host factor TOM1 inhibits tomato mosaic virus propagation and suppression of RNA silencing
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DOI：
10.1016/j.virol.2008.03.020
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发表时间：
2008-06-20
期刊：
影响因子：
3.7
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
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作者：
Hagiwara-Komoda, Yuka;Hirai, Katsuyuki;Ishikawa, Masayuki
植物整体膜蛋白TOW参与番茄花叶病毒（ToMV）的增殖。TOM1与ToMV复制蛋白相互作用，并被认为将复制蛋白拴在病毒RNA合成发生的膜上。我们之前已经证明TOM1的失活导致ToMV增殖减少。在本研究中，我们发现烟草中TOM1的过表达也会抑制ToMV的繁殖。TOM1过表达导致复制蛋白可溶性形式的积累减少，并干扰复制蛋白抑制RNA沉默的能力。在沉默抑制缺陷的ToMV突变体中也观察到可溶性复制蛋白的积累减少。基于这些结果，我们提出RNA沉默抑制是由复制蛋白的可溶性形式执行的，有效的ToMV增殖需要可溶性和膜结合复制蛋白的平衡积累。(c) 2008爱思唯尔公司版权所有。
A plant integral membrane protein TOW is involved in the multiplication of Tomato mosaic virus (ToMV). TOM1 interacts with ToMV replication proteins and has been suggested to tether the replication proteins to the membranes where the viral RNA synthesis takes place. We have previously demonstrated that inactivation of TOM1 results in reduced ToMV multiplication. In the present study, we show that overexpression of TOM1 in tobacco also inhibits ToMV propagation. TOM1 overexpression led to a decreased accumulation of the soluble form of the replication proteins and interfered with the ability of the replication protein to suppress RNA silencing. The reduced accumulation of the soluble replication proteins was also observed in a silencing suppressor-defective ToMV mutant. Based on these results, we propose that RNA silencing suppression is executed by the soluble form of the replication proteins and that efficient ToMV multiplication requires balanced accumulation of the soluble and membrane-bound replication proteins. (c) 2008 Elsevier Inc. All rights reserved.