Development of the cutaneous microbiome in the preterm infant: A prospective longitudinal study
Development of the cutaneous microbiome in the preterm infant: A prospective longitudinal study
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DOI：
10.1371/journal.pone.0176669
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发表时间：
2017-04-27
期刊：
影响因子：
3.7
通讯作者：
Petrosino, Joseph F.
中科院分区：
文献类型：
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作者：
Pammi, Mohan;O'Brien, Jacqueline L.;Petrosino, Joseph F.
背景早产儿的新生儿败血症通常是由于细菌定植在皮肤上，包括葡萄球菌。和念珠菌属（Candida spp.）新生儿期皮肤微生物组的发育和成熟，特别是早产儿，可能对预防病原体定植和随后进展为新生儿败血症至关重要。早产儿皮肤微生物组的发展或其决定因素在前4周的生命还没有evaluated.MethodsWe评估了皮肤微生物组从三个身体部位，肘前窝，前额和臀区，在一个前瞻性队列的15个早产儿（出生体重< 1500 g和< 32周的妊娠）和15个足月新生儿。通过在454 GS FLX平台上对16 S rRNA基因的细菌V3-V5区域进行扩增和测序，评估微生物组群落成员和相对丰度。我们使用线性混合效应模型来分析纵向data.ResultsThe结构和组成的皮肤微生物组之间没有显着差异的三个采样站点的足月和早产儿在新生儿期。然而，皮肤细菌丰富度与出生后前四周的胎龄呈正相关。静脉注射抗生素对皮肤的细菌多样性产生负面影响，但我们没有看到喂养或分娩方式的差异。ConclusionsGeepage，影响皮肤结构和功能的成熟度，与早产儿皮肤微生物组的发展有关。了解健康皮肤微生物组的成熟，预防病原体定植及其在免疫力发展中的作用，将是开发预防重症早产儿感染的新型干预措施的关键。
BackgroundNeonatal sepsis in preterm infants is often due to organisms that colonize the skin including Staphylococcus spp. and Candida spp. Development and maturation of the skin microbiome in the neonatal period, especially in preterm infants, may be critical in preventing colonization with pathogens and subsequent progression to neonatal sepsis. Development of the skin microbiome in preterm infants or its determinants in the first 4 weeks of life has not been evaluated.MethodsWe evaluated the skin microbiome from three body sites, antecubital fossa, forehead and gluteal region, in a prospective cohort of 15 preterm (birth weight < 1500 g and < 32 weeks of gestation) and 15 term neonates. The microbiome community membership and relative abundance were evaluated by amplification and sequencing the bacterial V3-V5 region of the16S rRNA gene on the 454 GS FLX platform. We used linear mixed effects models to analyze longitudinal data.ResultsThe structure and composition of the skin microbiome did not differ between the three sampling sites for term and preterm infants in the neonatal period. However, skin bacterial richness was positively associated with gestational age in the first four weeks of life. Intravenous antibiotics negatively impacted the bacterial diversity of the skin but we did not see differences with respect to feeding or mode of delivery.ConclusionsGestational age, which influences the maturity of skin structure and function, is associated with the development of the preterm cutaneous microbiome. Understanding the maturation of a healthy skin microbiome, prevention of pathogen colonization and its role in the development of immunity will be pivotal in the development of novel interventions to prevent infections in critically ill preterm infants.