Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano
Unique clusters of Archaea in Salar de Huasco, an athalassohaline evaporitic basin of the Chilean Altiplano
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DOI：
10.1111/j.1574-6941.2010.00891.x
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发表时间：
2010-08-01
影响因子：
4.2
通讯作者：
Witzel, Karl-Paul
中科院分区：
文献类型：
--
作者：
Dorador, Cristina;Vila, Irma;Witzel, Karl-Paul
对智利高原阿塔拉索盐盐湖（Salar de Huasco）不同地点的水和沉积物的克隆文库进行分析，发现了五个独特的未培养古菌群的存在，这些群以前没有被报道过或被特别指定过。这些序列与有限数量的其他克隆序列有远缘关系（序列同源性为83-96%），与培养的古菌没有同源性。利用qPCR技术估计了古细菌和细菌的丰度，并通过构建古细菌16S rRNA基因克隆文库分析了群落组成。在两个盐碱地(H4: 6.31 × 10(4)和H6: 1.37 × 10（4) μ S cm(-1)）的沉积物和一个水样（H0: 607 μ S cm（-1）的淡水）中，发现古细菌比细菌占优势。euryarchaeo序列比crenarchaeo序列更丰富。许多克隆序列（52%）与在海洋生态系统中发现的未培养的古菌群相似，同源值在99%至97%之间。大部分序列（40%）属于甲烷菌门，而其他序列则属于海洋底栖动物群B和D、杂绿古菌群、陆生杂绿古菌群、海洋I群和盐菌门。在Huasco Salar de Huasco发现的未经培养的古细菌群扩展了它们在内陆水域的已知分布，为它们在环境中的可能功能提供了新的线索。
Analyses of clone libraries from water and sediments of different sites from Salar de Huasco, a high-altitude athalassohaline wetland in the Chilean Altiplano, revealed the presence of five unique clusters of uncultured Archaea that have not been previously reported or specifically assigned. These sequences were distantly related (83-96% sequence identity) to a limited number of other clone sequences and revealed no identity to cultured Archaea. The abundance of Archaea and Bacteria was estimated using qPCR and community composition was examined through the construction of clone libraries of archaeal 16S rRNA gene. Archaea were found to be dominant over Bacteria in sediments from two saline sites (sites H4: 6.31 x 10(4) and site H6: 1.37 x 10(4) mu S cm(-1)) and in one of the water samples (freshwater from site H0: 607 mu S cm(-1)). Euryarchaeotal sequences were more abundant than crenarchaeotal sequences. Many of the clone sequences (52%) were similar to uncultured archaeal groups found in marine ecosystems having identity values between 99% and 97%. A major fraction of the sequences (40%) were members of Methanobacteria, while others were included in the Marine Benthic Groups B and D, the Miscellaneous Crenarchaeotic Group, the Terrestrial Miscellaneous Euryarchaeotal Group, Marine Group I and Halobacteria. The presence of uncultured archaeal groups in Salar de Huasco extends their known distribution in inland waters, providing new clues about their possible function in the environment.