Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells.
Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells.
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连续递送 TGF-β 3 的丝素蛋白/软骨细胞外基质支架用于脂肪干细胞的软骨分化
DOI：
10.2147/ijn.s141888
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发表时间：
2017
影响因子：
8
通讯作者：
Zhao YH
中科院分区：
文献类型：
--
作者：
Yang Q;Teng BH;Wang LN;Li K;Xu C;Ma XL;Zhang Y;Kong DL;Wang LY;Zhao YH
模拟体内微环境以再生软骨的三维支架是理想的。在本研究中，我们将丝素蛋白和脱细胞软骨细胞外基质通过温度梯度引导的热诱导相分离制备复合支架（S/D）。所得支架具有良好的力学性能和仿生结构，适合脂肪干细胞（ADSCs）的贴壁和增殖。此外，负载于支架上的转化生长因子β3（transforminggrowthfactor β 3，TGF-β3）在28 d培养过程中表现出可控的释放特性，并促进ADSCs向软骨细胞的分化。S/D支架本身可以提供一种缓释系统，而无需引入其他控释介质，具有商业和临床应用的潜力。甲苯胺蓝，番红O，免疫组化染色和胶原II表达分析的结果表明，在28天的培养后，在所有支架的软骨形成表型的维护。最明显的现象是加入TGF-β3。序贯导入TGF-β3的S/D复合支架可模拟再生微环境，促进ADSCs体外向软骨细胞分化。
A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture. The S/D scaffold itself can provide a sustained release system without the introduction of other controlled release media, which has potential for commercial and clinical applications. The results of toluidine blue, Safranin O, and immunohistochemical staining and analysis of collagen II expression showed maintenance of a chondrogenic phenotype in all scaffolds after 28-day culture. The most obvious phenomenon was with the addition of TGF-β3. S/D composite scaffolds with sequential delivery of TGF-β3 may mimic the regenerative microenvironment to enhance the chondrogenic differentiation of ADSCs in vitro.