Interleukin-1 beta simultaneously affects the stress and reproductive axes by modulating norepinephrine levels in different brain areas.
Interleukin-1 beta simultaneously affects the stress and reproductive axes by modulating norepinephrine levels in different brain areas.
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Interleukin-1 beta 通过调节不同大脑区域的去甲肾上腺素水平同时影响压力轴和生殖轴。
DOI：
10.1016/j.lfs.2012.09.004
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
MohanKumar,ShebaMJ
中科院分区：
文献类型：
--
作者：
Sirivelu,MadhuP;MohanKumar,PS;MohanKumar,ShebaMJ
白介素1受体(IL-1β，IL-1β)是一种激活应激轴、抑制生殖轴的细胞因子。不同的大脑区域参与了这两个轴的调节。然而，它们都受到儿茶酚胺、去甲肾上腺素(NE)的刺激控制。在这里，我们假设IL-1β通过调节特定脑区的NE水平来不同地影响这两个轴。方法发情前期的SD大鼠在下午1点给予PBS-1.0%牛血清白蛋白(对照组)或5μg的IL-1β。分别于下午1、3、5时处死大鼠，收集脑组织。分离与生殖相关的大脑区域以及与应激轴活动相关的区域，并使用高效液相色谱-电化学法分析去甲肾上腺素浓度。分析了躯干血中IL-1β、皮质酮和黄体生成素水平。KEY FINDING是一个总体趋势，与对照组相比，IL-1β治疗显著降低了生殖功能控制区的NE水平(p<；0.05)。相反，应激相关区域的NE水平显著升高(p<；0.05)。IL-1β可显著降低大鼠体内促黄体生成素水平，显著提高皮质酮水平。IL-1β对应激和生殖轴产生不同作用的能力可以通过调节与这些功能相关的特定脑区的去甲肾上腺素水平来解释。这种对去甲肾上腺素的不同调节可能是一种适应现象，以应对全身免疫挑战。
AIMSInterleukin-1β (IL-1β) is a cytokine that is known to activate the stress axis and suppress the reproductive axis. Different brain areas are involved in the regulation of these two axes. However, they are both under the stimulatory control of the catecholamine, norepinephrine (NE). Here, we hypothesized that IL-1β differentially affects these two axes by modulating NE levels in specific brain regions.MAIN METHODSFemale Sprague–Dawley rats in proestrus were injected intraperitoneally with either PBS-1.0% BSA (control) or 5μg of IL-1β at 1pm. Groups of rats were sacrificed at 1, 3, and 5pm and their brains were collected. Brain areas associated with reproduction as well as areas associated with stress axis activity were isolated and analyzed for NE concentrations using HPLC–EC. Trunk blood was analyzed for IL-1β, corticosterone and luteinizing hormone levels.KEY FINDINGSAs a general trend, treatment with IL-1β significantly decreased NE levels (p<0.05) in the areas controlling reproductive functions when compared to the control group. In contrast, NE levels increased significantly (p<0.05) in the stress associated areas. LH levels were markedly decreased with IL-1β treatment while corticosterone levels increased dramatically.SIGNIFICANCEThe ability of IL-1β to produce differential effects on the stress and reproductive axis could be explained by modulation of NE levels in specific brain areas that are associated with these functions. This differential regulation of NE may be an adaptive phenomenon in response to a systemic immune challenge.