Regulation of transcriptional bursting by core promoter elements in the Drosophila embryo
Regulation of transcriptional bursting by core promoter elements in the Drosophila embryo
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DOI：
10.1101/2021.03.18.435761
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
Moe Yokoshi;M. Cambón;Takashi Fukaya
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文献类型：
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作者：
Moe Yokoshi;M. Cambón;Takashi Fukaya
转录爆发是一种普遍存在的跨物种保守基因表达的特征，但其机制知之甚少。在这里，我们提供的证据表明，核心启动子元件调节转录爆发在早期果蝇胚胎的多个水平。定量实时成像分析显示，下游核心启动子元件有助于调节爆发频率。相反，上游TATA盒主要影响转录爆发的幅度。基因组编辑分析进一步揭示了内源性TATA盒和下游核心启动子元件的爆发调控对于关键发育基因的空间模式化是必不可少的。我们认为，核心启动子元件作为一个关键的调节模块，在动物发育过程中转换增强子活性的转录爆发。
Transcriptional bursting is a pervasive feature of gene expression conserved across species, but its mechanism is poorly understood. Here, we provide evidence that core promoter elements regulate transcriptional bursting at multiple levels in early Drosophila embryos. Quantitative live imaging analysis revealed that downstream core promoter elements contribute to regulation of bursting frequency. In contrast, the upstream TATA box mainly impacts amplitude of transcriptional bursting. Genome editing analysis further revealed that burst regulation by endogenous TATA box and downstream core promoter elements is essential for the spatial patterning of key developmental genes. We suggest that core promoter elements serve as a key regulatory module in converting enhancer activity into transcriptional bursting during animal development.