Levels of surfactant-associated protein messenger ribonucleic acids in rabbit lung during perinatal development and after hormonal treatment.
Levels of surfactant-associated protein messenger ribonucleic acids in rabbit lung during perinatal development and after hormonal treatment.
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围产期发育期间和激素治疗后兔肺中表面活性剂相关蛋白信使核糖核酸的水平。
作者：
Ian Connelly;G. Hammond;P. Harding;F. Possmayer
The levels of messenger RNAs for the surfactant-associated proteins, SP-A, SP-B, and SP-C, have been examined in the developing rabbit lung in vivo. Northern blot analysis detected SP-C mRNA by day 22 of gestation (term 31 days) and SP-A mRNA and SP-B mRNA on day 26, while solution hybridization assays detected all three mRNAs on day 22 of gestation. Both techniques revealed that the mRNA levels increased rapidly during the last quarter of gestation. The mRNA levels determined by solution hybridization were highly correlated during development, with average molar ratios of 1.0:1.1:2.1 for SP-A, SP-B, and SP-C, respectively. We also examined the effect of accelerating fetal pulmonary maturation by maternal administration of either 17 beta-estradiol or betamethasone (9 alpha-fluoro-16 beta-methylprednisolone) on day 26 of gestation. These treatments increased SP-A mRNA levels 8- to 12-fold, resulting in levels 3- to 4-fold greater than in the adult. SP-B mRNA levels increased by approximately 2-fold to near adult levels, while SP-C mRNA was lowered somewhat by 17 beta-estradiol and significantly to less than half by betamethasone. No differences in the levels of surfactant apoprotein mRNAs or in choline incorporation into total or disaturated phosphatidylcholine were noted between male and female fetuses. These observations are consistent with the accepted view that the genes for the surfactant-associated proteins are independently regulated. However, the various factors affecting these mRNAs result in a coordination of mRNA levels during normal perinatal development.
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糖皮质激素对主要表面活性剂相关蛋白（SP-A）及其信使核糖核酸的调节以及人胎肺体外形态发育的调节。
影响因子：
4.8
作者：
Odom,MJ;Snyder,JM;Boggaram,V;Mendelson,CR
通讯作者：
Mendelson,CR
脐带血清中的糖皮质激素活性：氢化可的松和倍他米松方案的比较。
DOI：
10.1016/s0022-3476(82)80091-7
发表时间：
1982
期刊：
The Journal of pediatrics
影响因子：
--
作者：
Ballard,PL;Liggins,GC
通讯作者：
Liggins,GC
DOI：
10.1016/s0021-9258(18)69159-8
发表时间：
1988-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
V. Boggaram;K. Qing;C. Mendelson
通讯作者：
V. Boggaram;K. Qing;C. Mendelson
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Post,M;Barsoumian,A;Smith,BT
通讯作者：
Smith,BT
发育中的大鼠肺中表面活性剂组分脂质、蛋白质和生物物理特性之间的关系。
DOI：
10.1016/0005-2760(90)90222-j
发表时间：
1990
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Farrell,PM;Bourbon,JR;Notter,RH;Marin,L;Nogee,LM;Whitsett,JA
通讯作者：
Whitsett,JA