Violation of allelic exclusion of the T cell receptor beta genes in a helper T cell clone.
Violation of allelic exclusion of the T cell receptor beta genes in a helper T cell clone.
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DOI：
10.1093/intimm/1.3.273
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发表时间：
1989-01-01
影响因子：
4.4
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Schittek, B;Unkelbach, E;Rajewsky, K
4-羟基-3-硝基苯乙酰偶联于鸡丙种球蛋白特异性和I-Ab限制性辅助T细胞克隆IH4，一条染色体上有Vβ8.1-Dβ2.1-Jβ2.3-重排，另一条染色体上有Vβ16-Dβ2.1-Jβ2.5-重排。两个重排的β基因都被转录，两个基因的产物都在细胞表面表达。这一结果暗示在这个T细胞克隆中不存在T细胞受体β基因的等位基因排斥。Vβ16基因功能前导外显子5‘端的假性先导的发现表明该基因没有被有效翻译，合成的Vβ16链不足以激活阻止第二次Vβ到DβJβ重排的抑制机制。
The 4-hydroxy-3-nitrophenylacetyl coupled to chicken gamma globulin specific and I-Ab restricted helper T cell clone IH4 carries a V beta 8.1-D beta 2.1-J beta 2.3-rearrangement on one and a V beta 16-D beta 2.1-J beta 2.5-rearrangement on the other chromosome. Both rearranged beta genes are transcribed and the products of both genes are expressed on the cell surface. This result implicates the absence of allelic exclusion of the T cell receptor beta genes in this T cell clone. The finding of a pseudoleader 5' to the functional leader exon of the V beta 16 gene suggests that this gene is not efficiently translated and that the amount of V beta 16 chains synthesized is not sufficient to activate the inhibitory mechanism preventing a second V beta to D beta J beta rearrangement.