Comparison of Immune Responses to the O-Specific Polysaccharide and Lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi Adult Patients with Cholera
Comparison of Immune Responses to the O-Specific Polysaccharide and Lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi Adult Patients with Cholera
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DOI：
10.1128/cvi.00321-12
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发表时间：
2012-11-01
影响因子：
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通讯作者：
Ryan, Edward T.
中科院分区：
文献类型：
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作者：
Johnson, Russell A.;Uddin, Taher;Ryan, Edward T.
针对霍乱弧菌O 1的免疫是血清群特异性的，血清群由脂多糖（LPS）的O-特异性多糖（OSP）部分定义。尽管如此，人类对霍乱弧菌OSP的免疫应答先前尚未被表征。我们使用O 1 OSP-核心-牛血清白蛋白（OSPc：BSA）缀合物评估了孟加拉国达卡市霍乱弧菌O 1 El Tor血清型Inaba或Ogawa引起的霍乱成人对霍乱弧菌OSP的免疫应答;这些缀合物中的应答靶向OSP。从急性期到恢复期，稻叶型感染者对稻叶型OSP和LPS的IgG、IgM和伊加亚型的应答均显著增加，且OSP和LPS的应答相关性良好（R分别为0.86、0.73和0.91; P < 0.01）。Ogawa感染患者对Ogawa OSP和LPS的血浆IgG、IgM和伊加反应也彼此良好相关（R分别为0.60、0.60和0.92; P < 0.01）。对Inaba OSP和Ogawa OSP的血浆IgM应答与各自血清群特异性杀弧菌抗体相关（R分别= 0.80和0.66; P < 0.001）。添加OSPc：BSA或LPS，但不是BSA，杀弧菌测定抑制杀弧菌反应在一个相当的和浓度依赖性的方式。对OSP和LPS的粘液伊加免疫应答也相似。我们的研究首次表征了霍乱患者的抗OSP免疫应答，并表明靶向霍乱弧菌LPS的应答，包括与霍乱保护相关的杀弧菌应答，主要靶向OSP。抗OSP应答的诱导可能与针对霍乱的保护有关，并且我们的结果可能支持靶向霍乱弧菌OSP的疫苗的开发。
Immunity against Vibrio cholerae O1 is serogroup specific, and serogrouping is defined by the O-specific polysaccharide (OSP) part of lipopolysaccharide (LPS). Despite this, human immune responses to V. cholerae OSP have not previously been characterized. We assessed immune responses against V. cholerae OSP in adults with cholera caused by V. cholerae O1 El Tor serotype Inaba or Ogawa in Dhaka, Bangladesh, using O1 OSP-core-bovine serum albumin (OSPc:BSA) conjugates; responses targeted OSP in these conjugates. Responses of Inaba-infected patients to Inaba OSP and LPS increased significantly in IgG, IgM, and IgA isotypes from the acute to convalescent phases of illness, and the responses correlated well between OSP and LPS (R = 0.86, 0.73, and 0.91, respectively; P < 0.01). Plasma IgG, IgM, and IgA responses to Ogawa OSP and LPS in Ogawa-infected patients also correlated well with each other (R = 0.60, 0.60, and 0.92, respectively; P < 0.01). Plasma IgM responses to Inaba OSP and Ogawa OSP correlated with the respective serogroup-specific vibriocidal antibodies (R = 0.80 and 0.66, respectively; P < 0.001). Addition of either OSPc: BSA or LPS, but not BSA, to vibriocidal assays inhibited vibriocidal responses in a comparable and concentration-dependent manner. Mucosal IgA immune responses to OSP and LPS were also similar. Our study is the first to characterize anti-OSP immune responses in patients with cholera and suggests that responses targeting V. cholerae LPS, including vibriocidal responses that correlate with protection against cholera, predominantly target OSP. Induction of anti-OSP responses may be associated with protection against cholera, and our results may support the development of a vaccine targeting V. cholerae OSP.