Murine calvaria-derived progenitor cells express high levels of osterix and lose their adipogenic capacity.
Murine calvaria-derived progenitor cells express high levels of osterix and lose their adipogenic capacity.
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DOI：
10.1016/j.bbrc.2012.04.155
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发表时间：
2012-06
影响因子：
3.1
通讯作者：
Dahu Li;Chao Liang;Liang Xu;Chunyan Tian;G. Xing;F. He;Lingqiang Zhang
中科院分区：
文献类型：
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作者：
Dahu Li;Chao Liang;Liang Xu;Chunyan Tian;G. Xing;F. He;Lingqiang Zhang
虽然小鼠是最广泛使用的生物医学动物模型，但由于造血细胞的污染，难以从骨髓中分离小鼠间充质干细胞（MSCs）。小鼠长骨的密质骨组织被认为是一种新的和可靠的骨髓间充质干细胞来源，具有低造血细胞污染。我们研究了小鼠颅骨密质骨是否由于其低骨髓含量而成为有希望的MSC来源。我们用同样的方法从长骨和头盖骨中分离细胞。虽然它们具有与长骨来源的MSC相似的形态特征和表面抗原，但颅骨来源的细胞高度表达成骨转录因子osterix，失去了成脂能力并获得了更高的成骨能力。这些发现表明，从颅骨迁移的细胞是祖细胞，而不是骨髓间充质干细胞/祖细胞存在于不同的小鼠密质骨沉积的分化命运已经提交。
Though the mouse is the most widely used biomedical animal model, it is difficult to isolate murine mesenchymal stem cells (MSCs) from the bone marrow because of contamination by hematopoietic cells. The murine compact bone tissue of long bones is considered a novel and reliable source of MSCs with low hematopoietic cell contamination. We investigated whether the murine compact bone of the calvaria would be a promising source of MSCs due to its low bone marrow content. We isolated cells from both long bones and the calvaria using the same method. Although they shared morphological features and surface antigens similar to those of long bone-derived MSCs, the calvaria-derived cells highly expressed the osteogenic transcription factor osterix, lost their adipogenic capacity and gained a higher osteogenic capacity. These findings suggest that the cells that migrated from the calvaria were progenitor cells rather than MSCs and that the differentiation fate of mesenchymal stem/progenitor cells existing in different murine compact bone deposits is already committed.