Conversion from long-term cultivated wheat field to Jerusalem artichoke plantation changed soil fungal communities.
Conversion from long-term cultivated wheat field to Jerusalem artichoke plantation changed soil fungal communities.
复制标题
从长期耕种的麦田到菊芋种植园的转变改变了土壤真菌群落
DOI：
10.1038/srep41502
复制
发表时间：
2017-01-30
影响因子：
4.6
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Zhou X;Zhang J;Gao D;Gao H;Guo M;Li L;Zhao M;Wu F
了解农业生态系统中的土壤微生物群落有可能有助于提高农业生产力和可持续性。通过总真菌 ITS 区域的扩增子测序确定从长期小麦种植园转变为菊芋 (JA) 种植园对土壤真菌群落的影响。定量 PCR 和 PCR 变性梯度凝胶电泳也用于分析总真菌和木霉属。 ITS 区域和镰刀菌属。 Ef1α基因。结果表明，第一茬JA土壤有机碳较高，第三茬JA土壤有机碳较低。种植园转变改变了土壤总真菌和镰刀菌，但没有改变木霉属。群落结构和组成。第三季 JA 的总真菌群落多样性和镰刀菌属最低。群落丰度较高，但真菌和木霉总数最高。社区丰富性。麦田中小麦潜在真菌病原菌的相对丰度较高。具有促进植物生长、植物病原体或昆虫拮抗潜力的真菌类群在JA的第一和第二季中得到富集。总体而言，从小麦到JA种植园的短期转变改变了土壤真菌群落，这与土壤有机碳和奥尔森磷含量的变化有关。
Understanding soil microbial communities in agroecosystems has the potential to contribute to the improvement of agricultural productivity and sustainability. Effects of conversion from long-term wheat plantation to Jerusalem artichoke (JA) plantation on soil fungal communities were determined by amplicon sequencing of total fungal ITS regions. Quantitative PCR and PCR-denaturing gradient gel electrophoresis were also used to analyze total fungal and Trichoderma spp. ITS regions and Fusarium spp. Ef1α genes. Results showed that soil organic carbon was higher in the first cropping of JA and Olsen P was lower in the third cropping of JA. Plantation conversion changed soil total fungal and Fusarium but not Trichoderma spp. community structures and compositions. The third cropping of JA had the lowest total fungal community diversity and Fusarium spp. community abundance, but had the highest total fungal and Trichoderma spp. community abundances. The relative abundances of potential fungal pathogens of wheat were higher in the wheat field. Fungal taxa with plant growth promoting, plant pathogen or insect antagonistic potentials were enriched in the first and second cropping of JA. Overall, short-term conversion from wheat to JA plantation changed soil fungal communities, which is related to changes in soil organic carbon and Olsen P contents.