Yield reduction historically associated with the Aegilops ventricosa 7DV introgression is genetically and physically distinct from the eyespot resistance gene Pch1.
Yield reduction historically associated with the Aegilops ventricosa 7DV introgression is genetically and physically distinct from the eyespot resistance gene Pch1.
复制标题
历史上与 Aegilops ventricosa 7DV 基因渗入相关的产量降低在遗传和物理上与眼斑抗性基因 Pch1 不同。
DOI：
10.1007/s00122-019-03502-1
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Pasquariello M
中科院分区：
文献类型：
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作者：
Pasquariello M
与偏凸山羊草7D渐渗相关的产量损失和增加的籽粒蛋白质含量性状已首次被定位，并且它们在物理上不同于眼斑病抗性位点Pch 1。Pch 1是一个显性基因，它赋予小麦抗眼斑病的能力，并作为7DV染色体的一个大片段渗入到偏凸山羊草的小麦中。这种基因渗入与显著的产量降低和伴随的谷物蛋白质含量的增加有关。在这项研究中，我们评估了这两个性状及其与Pch1基因位置的关系。我们发现这两个QTL都明显不同于Pch 1基因，位于与Pch 1不同的连锁群。此外，我们发现，增加籽粒蛋白质含量的QTL是强大的和一致的田间试验，而产量惩罚QTL是不稳定的和环境依赖性。产量和籽粒蛋白质含量QTL之间存在遗传连锁，且位于同一连锁群中。这一发现部分是由于群体规模小，以及小麦7D和Ae之间的限制性重组。心室7Dv染色体。虽然在这段时间里重组是罕见的，但它确实发生了。鉴定了一个含有Pch 1和7D_KASP 6（与籽粒蛋白质含量增加相关的标记）但不含Xwmc 221（与产量惩罚效应相关的标记）的重组系。
Key messageYield penalty and increased grain protein content traits associated withAegilops ventricosa7D introgression have been mapped for the first time, and they are physically distinct from the eyespot resistance locusPch1.AbstractWheat wild relatives represent an important source of genetic variation, but introgression of agronomically relevant genes, such as for disease resistance, may lead to the simultaneous introduction of genetically linked deleterious traits.Pch1is a dominant gene, conferring resistance to eyespot and was introgressed to wheat fromAegilops ventricosaas part of a large segment of the 7DVchromosome. This introgression has been associated with a significant yield reduction and a concomitant increase in grain protein content. In this study, we evaluated both traits and their relationship to the location of thePch1gene. We found that both QTLs were clearly distinct from thePch1gene, being located on a different linkage group toPch1. In addition, we found that the QTL for increased grain protein content was strong and consistent across field trials, whereas the yield penalty QTL was unstable and environmentally dependent. The yield and grain protein content QTLs were genetically linked and located in the same linkage group. This finding is due in part to the small size of the population, and to the restricted recombination between wheat 7D andAe. ventricosa7Dvchromosomes. Although recombination in this interval is rare, it does occur. A recombinant line containingPch1and7D_KASP6, the marker associated with increase in grain protein content, but notXwmc221, the marker associated with the yield penalty effect, was identified.