Author Correction: An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells
Author Correction: An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells
复制标题
作者更正：一种光化学工具，用于光诱导粘附连接解离，以控制细胞之间的机械耦合
DOI：
10.1038/s41467-020-15275-z
复制
发表时间：
2020
影响因子：
16.6
通讯作者：
Ollech D
中科院分区：
文献类型：
--
作者：
Ollech D
粘附连接处的钙粘蛋白-连环蛋白复合物（AJs）对于细胞-细胞粘附和上皮完整性的形成至关重要；然而，在高时空分辨率下研究AJs的动态调节仍然具有挑战性。在这里，我们提出了一种光化学工具，可以通过化学二聚化的力承载结构和它们的精确光诱导解离来重建AJs。对于二聚化，我们通过直接募集α-catenin和将其细胞质尾部连接到跨膜结构域两种方式重建了无尾E-cadherin的肌动蛋白连接。我们的方法为细胞之间的机械耦合提供了一个特定的on - off开关，可以通过光切割在亚细胞或组织尺度上进行空间控制。结合细胞迁移分析和牵引力显微镜显示了广泛的适用性，并证实了重建AJs的力学贡献。值得注意的是，在体内，我们的工具能够控制发育中的爪蟾胚胎表皮层的结构和功能完整性。
The cadherin-catenin complex at adherens junctions (AJs) is essential for the formation of cell-cell adhesion and epithelium integrity; however, studying the dynamic regulation of AJs at high spatio-temporal resolution remains challenging. Here we present an optochemical tool which allows reconstitution of AJs by chemical dimerization of the force bearing structures and their precise light-induced dissociation. For the dimerization, we reconstitute acto-myosin connection of a tailless E-cadherin by two ways: direct recruitment of α-catenin, and linking its cytosolic tail to the transmembrane domain. Our approach enables a specific ON-OFF switch for mechanical coupling between cells that can be controlled spatially on subcellular or tissue scale via photocleavage. The combination with cell migration analysis and traction force microscopy shows a wide-range of applicability and confirms the mechanical contribution of the reconstituted AJs. Remarkably, in vivo our tool is able to control structural and functional integrity of the epidermal layer in developingXenopusembryos.