Genome-Wide Transcriptome Profiling of Mycobacterium smegmatis MC² 155 Cultivated in Minimal Media Supplemented with Cholesterol, Androstenedione or Glycerol.
Genome-Wide Transcriptome Profiling of Mycobacterium smegmatis MC² 155 Cultivated in Minimal Media Supplemented with Cholesterol, Androstenedione or Glycerol.
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在补充有胆固醇、雄烯二酮或甘油的基本培养基中培养的耻垢分枝杆菌 MC² 155 的全基因组转录组分析。
DOI：
10.3390/ijms17050689
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发表时间：
2016-05-07
影响因子：
5.6
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Li Q;Ge F;Tan Y;Zhang G;Li W
耻垢分枝杆菌MC2 155是研究耻垢分枝杆菌的理想模式菌。结核病和其他分枝杆菌病原体，因为它可以利用胆固醇作为碳资源生长良好。然而，其全球转录组学反应在很大程度上仍然未被揭示。在本研究中，M.分别收集在雄烯二酮、胆固醇和甘油补充的培养基中培养的smeglycerMC2155用于RNA测序研究。结果表明，在添加雄烯二酮、胆固醇和甘油的培养基中，有6004、6681和6348个基因表达，其中5891个基因在三种培养基中均得到表达，其中237个基因在添加胆固醇的培养基中特异表达。与其他两种补充剂相比，胆固醇共显著上调了1852和454个基因。只有偶尔的变化，观察到在基本的碳和氮代谢，而几乎所有的基因参与胆固醇catalysts和哺乳动物细胞进入（MCE）的上调胆固醇，但不是雄烯二酮。与甘油或雄烯二酮相比，胆固醇分别诱导了11个和16个基因簇。这项研究提供了一个全面的分析胆固醇反应转录组的M。恶臭我们的研究结果表明，胆固醇诱导更多的基因，并增加了大部分基因的表达参与胆固醇降解和MCE在M。而雄烯二酮没有同样的效果。
Mycobacterium smegmatis strain MC2 155 is an attractive model organism for the study of M. tuberculosis and other mycobacterial pathogens, as it can grow well using cholesterol as a carbon resource. However, its global transcriptomic response remains largely unrevealed. In this study, M. smegmatis MC2 155 cultivated in androstenedione, cholesterol and glycerol supplemented media were collected separately for a RNA-Sequencing study. The results showed that 6004, 6681 and 6348 genes were expressed in androstenedione, cholesterol and glycerol supplemented media, and 5891 genes were expressed in all three conditions, with 237 specially expressed in cholesterol added medium. A total of 1852 and 454 genes were significantly up-regulated by cholesterol compared with the other two supplements. Only occasional changes were observed in basic carbon and nitrogen metabolism, while almost all of the genes involved in cholesterol catabolism and mammalian cell entry (MCE) were up-regulated by cholesterol, but not by androstenedione. Eleven and 16 gene clusters were induced by cholesterol when compared with glycerol or androstenedione, respectively. This study provides a comprehensive analysis of the cholesterol responsive transcriptome of M. smegmatis. Our results indicated that cholesterol induced many more genes and increased the expression of the majority of genes involved in cholesterol degradation and MCE in M. smegmatis, while androstenedione did not have the same effect.