T-lymphocytes expressing CC chemokine receptor-5 are increased in frail older adults
T-lymphocytes expressing CC chemokine receptor-5 are increased in frail older adults
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DOI：
10.1111/j.1532-5415.2008.01673.x
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发表时间：
2008-05-01
影响因子：
6.3
通讯作者：
Leng, Sean X.
中科院分区：
文献类型：
--
作者：
De Fanis, Umberto;Wang, George C.;Leng, Sean X.
目的：评估表达CC趋化因子受体-5 （CCR5(+) t细胞）的t淋巴细胞的频率及其与老年人虚弱的关系。设计：年龄、种族和性别匹配的病例对照研究。单位：普通临床研究中心。参与者：来自马里兰州巴尔的摩市的72岁及以上的社区居民。方法：虚弱是通过5个有效的标准来确定的：虚弱、步行速度慢、疲劳、低体力活动和体重减轻。符合这五项标准中的三项或三项以上的人被定义为体弱，而不符合的人被定义为非体弱。使用全自动（Coulter）计数器进行全血细胞计数以获得外周血淋巴细胞计数。采集外周血进行CCR5和其他t细胞标记物的表面免疫荧光染色。结果：26名体弱和匹配的非体弱参与者（平均年龄+/-标准差83.8 +/- 5.3，范围72-94）完成了研究。体弱参与者的CCR5（+）、CCR5(+)CD8（+）和CCR5(+)CD45RO(-) t细胞计数高于匹配的非体弱对照组(349 +/- 160/mm(3) vs 194 +/- 168/mm(3)， P= 0.02；208 +/- 98/mm(3) vs 105 +/- 62/mm(3)， P= 0.02；189 +/- 149/mm(3) vs 52 +/- 36/mm(3)， P= 0.01；分别)。此外，在虚弱的参与者中，这些t细胞计数有逐渐增加的趋势(例如，CCR5(+)CD8（+）计数分别为123 +/- 52/mm(3), 248 +/- 115/mm（3)和360 +/- 215/mm(3)对于那些虚弱评分分别为3,4和5）。结论：这些初步结果表明CCR5(+) t细胞亚群在衰弱中扩增。它们为进一步表征CCR5(+) t细胞及其在虚弱中的作用提供了基础，具有潜在的治疗意义。
OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5(+) T-cells) and their relationship with frailty in older adults.DESIGN: Case-control study with an age-, race-, and sex-matched design.SETTING: General Clinical Research Center.PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland.METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers.RESULTS: Twenty-six frail and matched nonfrail participants (mean age +/- standard deviation 83.8 +/- 5.3, range 72-94) completed the study. Frail participants had higher CCR5(+), CCR5(+)CD8(+), and CCR5(+)CD45RO(-) T-cell counts than matched nonfrail controls (349 +/- 160/mm(3) vs 194 +/- 168/mm(3), P=.02; 208 +/- 98/mm(3) vs 105 +/- 62/mm(3), P=.02; and 189 +/- 149/mm(3) vs 52 +/- 36/mm(3), P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5(+)CD8(+) counts of 123 +/- 52/mm(3), 248 +/- 115/mm(3), and 360 +/- 215/mm(3) for those with frailty scores of 3, 4, and 5, respectively).CONCLUSION: These initial results suggest an expansion of the CCR5(+) T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5(+) T-cells and their role in frailty, with potential therapeutic implications.