Effects of selenium supplementation on antioxidant Defense and glucose Homeostasis in experimental diabetes Mellitus
Effects of selenium supplementation on antioxidant Defense and glucose Homeostasis in experimental diabetes Mellitus
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DOI：
10.1007/s12011-007-0037-5
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发表时间：
2007-09-01
影响因子：
3.9
通讯作者：
Coker, Canan
中科院分区：
文献类型：
--
作者：
Erbayraktar, Zubeyde;Yflmaz, Osman;Coker, Canan
本研究旨在探讨不同形式的硒对实验性糖尿病大鼠抗氧化防御和葡萄糖代谢的影响。用链脲佐菌素（STZ）诱导的糖尿病大鼠，在基础饲料的基础上，经口胃给予硒酸钠（SS）或硒代蛋氨酸（SM）（2 μ mol Se/kg·d），共12周。每月评估全血葡萄糖水平、红细胞谷胱甘肽过氧化物酶（GSH-Px）活性、血浆硒和果糖胺水平。与基础测量值相比，所有糖尿病组的血浆Se水平均显著升高，SM组更为显著[p（SM 3/SM 0）=0.018]。在SS中，GSH-Px活性在第二个月结束时显著增加[p（SS 2/SS 0）=0.028]，而在SM中，该值在第三个月结束时较低[p（SM 3/SM 0）=0.018]，而未补充的糖尿病对照（DC）组，p（DC 3/DC 0）=0.012。仅DC组血糖显著升高。果糖胺在糖尿病各组逐渐升高，以DC和SS组显著。在第三个月结束时，SS组中观察到最高果糖胺水平，其显著高于SM组[p（SM/SS）=0.010]。总之，硒通过增加GSH-Px活性增强抗氧化防御，并且当以SM形式补充硒时，这种效果更加突出，这对葡萄糖稳态也产生了积极影响。
The objective of this study was to investigate the effects of different forms of Se supplementation on the antioxidant defense and glucose bomeostasis in experimental diabetes. Sodium selenate (SS) or selenomethionine (SM) were administered (2 mu mol Se kg(-1) day(-1)) via orogastric route to streptozotocine (STZ)-induced diabetic rats in addition to basal diet for 12 weeks. Glucose levels in whole blood, glutathione peroxidase (GSH-Px) activity in erythrocytes, Se and fructosamine levels in plasma were evaluated monthly. Plasma Se levels increased significantly in all diabetic groups compared to basal measurements, being more prominent in SM group [p(SM3/SM0)=0.018]. The increase in GSH-Px activities was significant at the end of the second month in SS [p(SS2/SS0)=0.028], whereas at the end of the third month in SM the value was lower [p(SM3/SM0)=0.018] and the unsupplemented diabetic control (DC) groups, p(DC3/DC0)=0.012. Glucose increased significantly only in DC group. Fructosamine increased gradually in all diabetic groups, being significant in DC and SS groups. At the end of the third month, highest fructosamine levels were observed in SS group, which were significantly higher than the SM group [p(SM/SS)=0.010]. In conclusion, Se augmented the antioxidant defense by increasing GSH-Px activity and this effect was more prominent when Se was supplemented as SM, which exerted positive effects also on glucose homeostasis.