Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium
Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium
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DOI：
10.1016/j.reprotox.2007.10.004
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Panda, Manoj K.
中科院分区：
文献类型：
--
作者：
Acharya, Usha R.;Mishra, Monalisa;Panda, Manoj K.
镉是一种潜在的环境污染物。在动物的不同器官中表现出细胞毒性效应。在本研究中，腹腔注射氯化镉(1 mg/kg体重)增加了瑞士小鼠睾丸的脂质过氧化反应，表明在治疗后5至8周内存在氧化应激。染镉组小鼠睾丸超氧化物歧化酶(SOD)、过氧化氢酶(CT)和过氧化物酶(PD)活性在处理后较空白对照组显著降低。此外，镉暴露的小鼠睾丸中抗坏血酸的含量也显著下降。经Cd处理后，精子畸形率显著增加，精子数显著减少。本研究旨在探讨补充维生素C和维生素E对染镉小鼠睾丸的影响。因此，染镉组小鼠分别注射维生素C和维生素E，以评估维生素C和维生素E对抗镉诱导的细胞毒性和其他表现的作用。镉致小鼠补充维生素C(10 mg/kg体重)和维生素E(100 mg/kg体重)可降低脂质过氧化作用，提高精子数谱，降低精子畸形率，提高上述抗氧化酶活性，并在一定程度上增加抗坏血酸浓度。维生素在减少氧化应激对镉处理的瑞士小鼠睾丸精子发生的相关影响方面的作用已有报道。(C)2007 Elsevier Inc.保留所有权利。
Cadmium (Cd) is a potential pollutant of the environment. It manifests cyto-toxic effects in different organs in animals. In the present study, intraperitoneal injection of CdCl2 (I mg/kg body weight) increased lipid peroxidation in Swiss mice testes indicating oxidative stress during 5th to 8th week of post-treatment. The enzymatic activity of superoxide dismutase (SOD), catalase (CT) and peroxidase (PD) were significantly decreased over the post-treatment phase in Cd-treated mice testes compared to vehicle controls. Further, ascorbic acid content also declined significantly in Cd-exposed mice testes. Following Cd treatment, a marked increase in sperm abnormality percentage and significant decrease in sperm count was observed. The purpose of the present study was to evaluate the effect of vitamins C and E supplementation on Cd-treated mice testes. Therefore, Cd-treated mice groups were injected with vitamins C and E, separately, to assess the effect of the vitamins in combating Cd-induced cytotoxicity and other manifestations. Supplementation of vitamin C (10 mg/kg body weight) and vitamin E (100 mg/kg body weight) to Cd-induced mice groups declined lipid peroxidation, increased sperm count profile, depressed the percentage of sperm abnormality, increased the activity of antioxidant enzymes mentioned above and also increased the concentration of ascorbic acid to a measurable extent. The role of vitamins in reducing oxidative stress-related effects on spermatogenesis in Cd-treated Swiss mice testes have been reported. (C) 2007 Elsevier Inc. All rights reserved.