Mutation of Gonadal soma-derived factor induces medaka XY gonads to undergo ovarian development
Mutation of Gonadal soma-derived factor induces medaka XY gonads to undergo ovarian development
复制标题
DOI：
10.1016/j.bbrc.2015.09.112
复制
发表时间：
2015-11-06
影响因子：
3.1
通讯作者：
Matsuda, Masaru
中科院分区：
文献类型：
--
作者：
Imai, Takuto;Saino, Kentaro;Matsuda, Masaru
雌雄异体物种具有发育成睾丸或卵巢的双能性腺。在性别由遗传因素决定的物种中，性别决定基因的表达是指导双潜能性腺发育的第一个线索。下游基因的后续表达诱导性腺发育成睾丸或卵巢。 TGF-β家族成员性腺体源性因子（Gsdf）被认为是硬骨鱼性腺发育的重要基因，从早期到成熟阶段，其在睾丸中的表达水平高于在卵巢中的表达水平。然而，有关该基因的功能信息很少。在这项研究中，我们使用类转录激活因子效应核酸酶（TALEN）靶向青鳉鱼 Oryzias latipes 中的 Gsdf 编码区，并研究了 Gsdf 突变体青鳉的表型。尽管正常和杂合的 XY 性腺发育为睾丸，但所有具有 Gsdf 纯合突变的 XY 性腺在早期发育阶段都发育为卵巢。然而，三分之二的 Gsdf 突变体 XY 性腺在成年阶段发育成睾丸。这些结果表明，虽然性腺在没有 Gsdf 的情况下可以发育成完整的睾丸，但 Gsdf 功能对于在早期发育阶段指导双潜能性腺至关重要。因此，Gsdf 是一种睾丸发育的内源性诱导剂，类似于主要的性别决定基因。 (C) 2015 Elsevier Inc. 保留所有权利。
Gonochoristic species have a bipotential gonad that develops into a testis or an ovary. In species whose sex is determined by a genetic factor, the expression of a sex-determining gene is the first cue that directs the development of a bipotential gonad. Subsequent expression of downstream genes induces the gonad to develop into a testis or an ovary. The TGF-beta family member Gonadal soma-derived factor (Gsdf) is thought to be an important gene for gonadal development in teleost fish, and it is expressed at higher levels in the testis than in the ovary from early to mature stages. However, there is little functional information about the gene. In this study, we targeted the Gsdf coding region in the medaka fish Oryzias latipes using transcription activator-like effector nucleases (TALENs) and studied the phenotypes of the Gsdf mutant medaka. Although normal and heterozygous XY gonads developed into a testis, all XY gonads with a homozygous mutation in Gsdf developed into an ovary at early developmental stages. However, two-thirds of Gsdf mutant XY gonads developed into testes in the adult stages. These results demonstrate that although a gonad can develop into a complete testis in the absence of Gsdf, Gsdf function is critical for directing the bipotential gonad at early developmental stages. Therefore, Gsdf is an endogenous inducer of testicular development similar to a master sex-determining gene. (C) 2015 Elsevier Inc. All rights reserved.